Results 21 - 24 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12703 | 3' | -52.1 | NC_003349.1 | + | 112743 | 0.68 | 0.942819 |
Target: 5'- cAGUuuuaaauCCAUUAAgGACGUCAUCAACAa -3' miRNA: 3'- -UCAcu-----GGUAGUUgCUGCGGUGGUUGU- -5' |
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12703 | 3' | -52.1 | NC_003349.1 | + | 114681 | 0.72 | 0.789756 |
Target: 5'- cGUGA-CAUUAACGACGCCGUCAAUc -3' miRNA: 3'- uCACUgGUAGUUGCUGCGGUGGUUGu -5' |
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12703 | 3' | -52.1 | NC_003349.1 | + | 119260 | 0.66 | 0.975565 |
Target: 5'- uGUGACCGUCAACaacaagaguCGCUACacaGAUAa -3' miRNA: 3'- uCACUGGUAGUUGcu-------GCGGUGg--UUGU- -5' |
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12703 | 3' | -52.1 | NC_003349.1 | + | 120518 | 0.74 | 0.689017 |
Target: 5'- cGUGGCUGUCGugaAUGCCGCCGACAa -3' miRNA: 3'- uCACUGGUAGUugcUGCGGUGGUUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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