Results 1 - 16 of 16 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12705 | 3' | -55 | NC_003349.1 | + | 20383 | 0.65 | 0.907551 |
Target: 5'- aGACGACGUacgacaUGUUgGCAGCUugGUCACUGa -3' miRNA: 3'- aUUGUUGCA------ACGA-CGUCGG--CGGUGGC- -5' |
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12705 | 3' | -55 | NC_003349.1 | + | 82222 | 0.66 | 0.894421 |
Target: 5'- ---gAACGUcuacgGCcGCcGCCGCCGCCu -3' miRNA: 3'- auugUUGCAa----CGaCGuCGGCGGUGGc -5' |
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12705 | 3' | -55 | NC_003349.1 | + | 48731 | 0.67 | 0.860581 |
Target: 5'- cGACAAUGUUGUUaaaaacaaucuaugcGCuGCCGgCGCCa -3' miRNA: 3'- aUUGUUGCAACGA---------------CGuCGGCgGUGGc -5' |
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12705 | 3' | -55 | NC_003349.1 | + | 16986 | 0.67 | 0.857411 |
Target: 5'- -cGCGAcCGUcGCcGguGuuGCCACCGa -3' miRNA: 3'- auUGUU-GCAaCGaCguCggCGGUGGC- -5' |
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12705 | 3' | -55 | NC_003349.1 | + | 26075 | 0.67 | 0.841047 |
Target: 5'- cUGACAcACGcaaaaUUGCguaauuUGCcGCCGCCGCCGc -3' miRNA: 3'- -AUUGU-UGC-----AACG------ACGuCGGCGGUGGC- -5' |
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12705 | 3' | -55 | NC_003349.1 | + | 16750 | 0.67 | 0.832559 |
Target: 5'- cGGCAcCGUcGC-GCAccGCCGCCGCCc -3' miRNA: 3'- aUUGUuGCAaCGaCGU--CGGCGGUGGc -5' |
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12705 | 3' | -55 | NC_003349.1 | + | 17780 | 0.67 | 0.832559 |
Target: 5'- aAAC-AUGUUGCUGUAGCCGaacggaucCCACa- -3' miRNA: 3'- aUUGuUGCAACGACGUCGGC--------GGUGgc -5' |
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12705 | 3' | -55 | NC_003349.1 | + | 100497 | 0.68 | 0.805964 |
Target: 5'- -uACAucCGUUGCUGguGUCGCCGaacUCGu -3' miRNA: 3'- auUGUu-GCAACGACguCGGCGGU---GGC- -5' |
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12705 | 3' | -55 | NC_003349.1 | + | 26167 | 0.69 | 0.768205 |
Target: 5'- gUAACGGC--UGCUGCuGCUGCgGCUGa -3' miRNA: 3'- -AUUGUUGcaACGACGuCGGCGgUGGC- -5' |
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12705 | 3' | -55 | NC_003349.1 | + | 59123 | 0.69 | 0.768205 |
Target: 5'- -cGCAACcau-CUGC-GCCGCCACCGc -3' miRNA: 3'- auUGUUGcaacGACGuCGGCGGUGGC- -5' |
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12705 | 3' | -55 | NC_003349.1 | + | 82389 | 0.69 | 0.768205 |
Target: 5'- aGGCGACGcucagGCgGCGGCgGCgGCCGu -3' miRNA: 3'- aUUGUUGCaa---CGaCGUCGgCGgUGGC- -5' |
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12705 | 3' | -55 | NC_003349.1 | + | 103615 | 0.69 | 0.758418 |
Target: 5'- -uACAAUccuGUUGCaauaUGCcgAGCCGCUACCGa -3' miRNA: 3'- auUGUUG---CAACG----ACG--UCGGCGGUGGC- -5' |
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12705 | 3' | -55 | NC_003349.1 | + | 116612 | 0.7 | 0.718185 |
Target: 5'- cGGCGGCGguggcgGCUGCAGCaacguuGUUACCGa -3' miRNA: 3'- aUUGUUGCaa----CGACGUCGg-----CGGUGGC- -5' |
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12705 | 3' | -55 | NC_003349.1 | + | 15610 | 0.7 | 0.697558 |
Target: 5'- -uACAugG-UGUcugUGUAGCCGCaCACCGa -3' miRNA: 3'- auUGUugCaACG---ACGUCGGCG-GUGGC- -5' |
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12705 | 3' | -55 | NC_003349.1 | + | 102702 | 0.82 | 0.152202 |
Target: 5'- --cUAACGUUGgUGCAGCCGCgACCGa -3' miRNA: 3'- auuGUUGCAACgACGUCGGCGgUGGC- -5' |
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12705 | 3' | -55 | NC_003349.1 | + | 116774 | 1.06 | 0.003732 |
Target: 5'- gUAACAACGUUGCUGCAGCCGCCACCGc -3' miRNA: 3'- -AUUGUUGCAACGACGUCGGCGGUGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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