Results 1 - 19 of 19 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12705 | 5' | -45.4 | NC_003349.1 | + | 52022 | 0.66 | 0.999979 |
Target: 5'- uGCGGauccGACGguuUGGCUAUGACGUccUGAg -3' miRNA: 3'- cUGCUa---CUGCa--AUUGAUGCUGCA--ACU- -5' |
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12705 | 5' | -45.4 | NC_003349.1 | + | 112991 | 0.66 | 0.999971 |
Target: 5'- aGGCGAUGAUGUacAUguugucuuauaUGCGACGUucacuUGAa -3' miRNA: 3'- -CUGCUACUGCAauUG-----------AUGCUGCA-----ACU- -5' |
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12705 | 5' | -45.4 | NC_003349.1 | + | 47256 | 0.67 | 0.99996 |
Target: 5'- uGGCGGUGGCG---ACgACGACGUa-- -3' miRNA: 3'- -CUGCUACUGCaauUGaUGCUGCAacu -5' |
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12705 | 5' | -45.4 | NC_003349.1 | + | 116615 | 0.67 | 0.99996 |
Target: 5'- cGGCGGUGGCGgcuGCaGCaACGUUGu -3' miRNA: 3'- -CUGCUACUGCaauUGaUGcUGCAACu -5' |
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12705 | 5' | -45.4 | NC_003349.1 | + | 64076 | 0.67 | 0.999946 |
Target: 5'- cGACGAcaaUGACGagGACgacgACGACGacGAa -3' miRNA: 3'- -CUGCU---ACUGCaaUUGa---UGCUGCaaCU- -5' |
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12705 | 5' | -45.4 | NC_003349.1 | + | 64884 | 0.67 | 0.999946 |
Target: 5'- -uCGAUGACGaauauuugaaUAACUACGAcacgcacaCGUUGAa -3' miRNA: 3'- cuGCUACUGCa---------AUUGAUGCU--------GCAACU- -5' |
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12705 | 5' | -45.4 | NC_003349.1 | + | 126763 | 0.67 | 0.999905 |
Target: 5'- uGCGGUGAUGUUAACgaaACGcGCGUc-- -3' miRNA: 3'- cUGCUACUGCAAUUGa--UGC-UGCAacu -5' |
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12705 | 5' | -45.4 | NC_003349.1 | + | 4184 | 0.68 | 0.999875 |
Target: 5'- aAUGAUGAUGUUAAUgaugAUGAUGUUa- -3' miRNA: 3'- cUGCUACUGCAAUUGa---UGCUGCAAcu -5' |
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12705 | 5' | -45.4 | NC_003349.1 | + | 96687 | 0.68 | 0.999837 |
Target: 5'- -uUGAUGGCGauaagUAACaUGCGGCGaUUGAa -3' miRNA: 3'- cuGCUACUGCa----AUUG-AUGCUGC-AACU- -5' |
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12705 | 5' | -45.4 | NC_003349.1 | + | 15913 | 0.68 | 0.999837 |
Target: 5'- aGACGAUGAUGaucgugaugaUUGAUUAUGACuUUGGu -3' miRNA: 3'- -CUGCUACUGC----------AAUUGAUGCUGcAACU- -5' |
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12705 | 5' | -45.4 | NC_003349.1 | + | 83824 | 0.68 | 0.999655 |
Target: 5'- cGACGAuuUGACGUcgcgaaGAUUGuuguCGACGUUGAc -3' miRNA: 3'- -CUGCU--ACUGCAa-----UUGAU----GCUGCAACU- -5' |
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12705 | 5' | -45.4 | NC_003349.1 | + | 104577 | 0.69 | 0.999316 |
Target: 5'- cGACGAUGuACG-UAGCgacgACGAgGUUGu -3' miRNA: 3'- -CUGCUAC-UGCaAUUGa---UGCUgCAACu -5' |
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12705 | 5' | -45.4 | NC_003349.1 | + | 82308 | 0.7 | 0.998955 |
Target: 5'- aGCGGUGGCGguaGACgagGCGGCGguagGAg -3' miRNA: 3'- cUGCUACUGCaa-UUGa--UGCUGCaa--CU- -5' |
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12705 | 5' | -45.4 | NC_003349.1 | + | 69818 | 0.7 | 0.99872 |
Target: 5'- -----cGAUGUUAACUaaaccgGCGGCGUUGAu -3' miRNA: 3'- cugcuaCUGCAAUUGA------UGCUGCAACU- -5' |
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12705 | 5' | -45.4 | NC_003349.1 | + | 1478 | 0.7 | 0.99773 |
Target: 5'- uGACGGUGGCGgaggaGGCgGCGGCGgagGAg -3' miRNA: 3'- -CUGCUACUGCaa---UUGaUGCUGCaa-CU- -5' |
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12705 | 5' | -45.4 | NC_003349.1 | + | 14753 | 0.71 | 0.99541 |
Target: 5'- uGACGAUGuugGUUAACUAgcgacgcugccguCGAUGUUGAc -3' miRNA: 3'- -CUGCUACug-CAAUUGAU-------------GCUGCAACU- -5' |
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12705 | 5' | -45.4 | NC_003349.1 | + | 4215 | 0.72 | 0.990422 |
Target: 5'- uGAUGAUGAUGUUAAUgAUGAUGUUa- -3' miRNA: 3'- -CUGCUACUGCAAUUGaUGCUGCAAcu -5' |
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12705 | 5' | -45.4 | NC_003349.1 | + | 42908 | 0.73 | 0.985715 |
Target: 5'- aGACGAUGACGacGACgACGACGa--- -3' miRNA: 3'- -CUGCUACUGCaaUUGaUGCUGCaacu -5' |
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12705 | 5' | -45.4 | NC_003349.1 | + | 116740 | 1.11 | 0.025672 |
Target: 5'- aGACGAUGACGUUAACUACGACGUUGAc -3' miRNA: 3'- -CUGCUACUGCAAUUGAUGCUGCAACU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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