Results 1 - 18 of 18 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12706 | 5' | -42.8 | NC_003349.1 | + | 1533 | 0.66 | 1 |
Target: 5'- uGAGGCGgCGGCGGUGG-CGgcggUAUUGg -3' miRNA: 3'- -UUUUGCaGCCGUUACUaGCa---AUAAUg -5' |
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12706 | 5' | -42.8 | NC_003349.1 | + | 82201 | 0.66 | 1 |
Target: 5'- cAGGCGUUcgGGCGGUGGUCGcag--ACg -3' miRNA: 3'- uUUUGCAG--CCGUUACUAGCaauaaUG- -5' |
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12706 | 5' | -42.8 | NC_003349.1 | + | 88178 | 0.67 | 0.999999 |
Target: 5'- cGAGUGUCGGCAAUG--CGUUAcaaUUACu -3' miRNA: 3'- uUUUGCAGCCGUUACuaGCAAU---AAUG- -5' |
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12706 | 5' | -42.8 | NC_003349.1 | + | 52766 | 0.67 | 0.999998 |
Target: 5'- cAAAAcCGUCGGCGAaGAUCGa------ -3' miRNA: 3'- -UUUU-GCAGCCGUUaCUAGCaauaaug -5' |
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12706 | 5' | -42.8 | NC_003349.1 | + | 125902 | 0.67 | 0.999997 |
Target: 5'- cAAAAUGUUGGCAAUaAUCGUUu---- -3' miRNA: 3'- -UUUUGCAGCCGUUAcUAGCAAuaaug -5' |
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12706 | 5' | -42.8 | NC_003349.1 | + | 35840 | 0.68 | 0.999993 |
Target: 5'- --cACGUUGGCGGUGuacgguUCGUUAg--- -3' miRNA: 3'- uuuUGCAGCCGUUACu-----AGCAAUaaug -5' |
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12706 | 5' | -42.8 | NC_003349.1 | + | 23309 | 0.68 | 0.999993 |
Target: 5'- uGAAAcCGUCGGCGAaGAUCGa------ -3' miRNA: 3'- -UUUU-GCAGCCGUUaCUAGCaauaaug -5' |
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12706 | 5' | -42.8 | NC_003349.1 | + | 92612 | 0.68 | 0.999993 |
Target: 5'- uGAAAcCGUCGGCGAaGAUCGa------ -3' miRNA: 3'- -UUUU-GCAGCCGUUaCUAGCaauaaug -5' |
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12706 | 5' | -42.8 | NC_003349.1 | + | 93278 | 0.68 | 0.999993 |
Target: 5'- uGAAAcCGUCGGCGAaGAUCGa------ -3' miRNA: 3'- -UUUU-GCAGCCGUUaCUAGCaauaaug -5' |
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12706 | 5' | -42.8 | NC_003349.1 | + | 58578 | 0.68 | 0.999986 |
Target: 5'- -uGACGUCGGuCAAcGAUCGUg----- -3' miRNA: 3'- uuUUGCAGCC-GUUaCUAGCAauaaug -5' |
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12706 | 5' | -42.8 | NC_003349.1 | + | 108635 | 0.69 | 0.999981 |
Target: 5'- uGAAAcCGUCGGCAAaGAUCGa------ -3' miRNA: 3'- -UUUU-GCAGCCGUUaCUAGCaauaaug -5' |
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12706 | 5' | -42.8 | NC_003349.1 | + | 33491 | 0.69 | 0.999973 |
Target: 5'- uGAGCGUgGGCGGauUGAuuucaaugUUGUUGUUGCu -3' miRNA: 3'- uUUUGCAgCCGUU--ACU--------AGCAAUAAUG- -5' |
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12706 | 5' | -42.8 | NC_003349.1 | + | 125898 | 0.7 | 0.999839 |
Target: 5'- aAAAACGcCGGCAAaauucGAUUGUUAgUACa -3' miRNA: 3'- -UUUUGCaGCCGUUa----CUAGCAAUaAUG- -5' |
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12706 | 5' | -42.8 | NC_003349.1 | + | 39636 | 0.71 | 0.999648 |
Target: 5'- gAAGACGaaGGCGGUGG-CGUUGUUGu -3' miRNA: 3'- -UUUUGCagCCGUUACUaGCAAUAAUg -5' |
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12706 | 5' | -42.8 | NC_003349.1 | + | 111634 | 0.73 | 0.998323 |
Target: 5'- -cGACGUCGGUGAUGucAUgGUUGUUAa -3' miRNA: 3'- uuUUGCAGCCGUUAC--UAgCAAUAAUg -5' |
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12706 | 5' | -42.8 | NC_003349.1 | + | 108347 | 0.75 | 0.993113 |
Target: 5'- uGAAAcCGUCGGCGAaGAUCG--AUUACa -3' miRNA: 3'- -UUUU-GCAGCCGUUaCUAGCaaUAAUG- -5' |
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12706 | 5' | -42.8 | NC_003349.1 | + | 35301 | 0.91 | 0.418523 |
Target: 5'- cAAAAUGUCGGCAAUGAUCGUUGg--- -3' miRNA: 3'- -UUUUGCAGCCGUUACUAGCAAUaaug -5' |
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12706 | 5' | -42.8 | NC_003349.1 | + | 35176 | 1.1 | 0.039782 |
Target: 5'- gAAAACGUCGGCAAUGAUCGUUAUUACa -3' miRNA: 3'- -UUUUGCAGCCGUUACUAGCAAUAAUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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