Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12707 | 3' | -50.5 | NC_003349.1 | + | 78868 | 0.66 | 0.992767 |
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Target: 5'- -aUACAcccUUGCUGcCGUCCGACAUGcGUa -3' miRNA: 3'- gcAUGU---AACGAU-GCGGGUUGUGCuCA- -5' |
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| 12707 | 3' | -50.5 | NC_003349.1 | + | 39094 | 0.66 | 0.98906 |
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Target: 5'- uGUACA--GC-AUGUCCAugACGCGAGUa -3' miRNA: 3'- gCAUGUaaCGaUGCGGGU--UGUGCUCA- -5' |
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| 12707 | 3' | -50.5 | NC_003349.1 | + | 91599 | 0.66 | 0.987541 |
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Target: 5'- gCGUACGUgacacugGUcAUGCUCGGCgACGAGUa -3' miRNA: 3'- -GCAUGUAa------CGaUGCGGGUUG-UGCUCA- -5' |
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| 12707 | 3' | -50.5 | NC_003349.1 | + | 58407 | 0.69 | 0.958402 |
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Target: 5'- uGUACaAUUGCaGCGCuUCGACACGAu- -3' miRNA: 3'- gCAUG-UAACGaUGCG-GGUUGUGCUca -5' |
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| 12707 | 3' | -50.5 | NC_003349.1 | + | 46812 | 0.71 | 0.886351 |
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Target: 5'- ---cUAUUGCUACgaaaGCCCAACACGAu- -3' miRNA: 3'- gcauGUAACGAUG----CGGGUUGUGCUca -5' |
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| 12707 | 3' | -50.5 | NC_003349.1 | + | 89463 | 0.72 | 0.855536 |
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Target: 5'- gGUAUAUgcGCUACGaCCAAUGCGGGUg -3' miRNA: 3'- gCAUGUAa-CGAUGCgGGUUGUGCUCA- -5' |
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| 12707 | 3' | -50.5 | NC_003349.1 | + | 98031 | 0.74 | 0.783763 |
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Target: 5'- aCGUaagaACAUUGCgGCGCCCAACguGCGuGUu -3' miRNA: 3'- -GCA----UGUAACGaUGCGGGUUG--UGCuCA- -5' |
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| 12707 | 3' | -50.5 | NC_003349.1 | + | 35772 | 1.08 | 0.00971 |
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Target: 5'- cCGUACAUUGCUACGCCCAACACGAGUu -3' miRNA: 3'- -GCAUGUAACGAUGCGGGUUGUGCUCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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