Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12709 | 3' | -54.9 | NC_003349.1 | + | 7742 | 0.66 | 0.93079 |
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Target: 5'- cGUCGUCUGUG-UCAUCGUUCaAACa -3' miRNA: 3'- cUAGCAGACGCuGGUGGCGAGcUUGc -5' |
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| 12709 | 3' | -54.9 | NC_003349.1 | + | 82224 | 0.68 | 0.866956 |
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Target: 5'- --aCGUCUaCGGCCGCCGCcgccgccUGAGCGu -3' miRNA: 3'- cuaGCAGAcGCUGGUGGCGa------GCUUGC- -5' |
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| 12709 | 3' | -54.9 | NC_003349.1 | + | 82185 | 0.68 | 0.835177 |
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Target: 5'- --gCGUC-GaCGACCGCCGCUaGAACu -3' miRNA: 3'- cuaGCAGaC-GCUGGUGGCGAgCUUGc -5' |
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| 12709 | 3' | -54.9 | NC_003349.1 | + | 82160 | 0.71 | 0.693768 |
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Target: 5'- -cUCGUCUaCcGCCACCGCUgGAACu -3' miRNA: 3'- cuAGCAGAcGcUGGUGGCGAgCUUGc -5' |
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| 12709 | 3' | -54.9 | NC_003349.1 | + | 127212 | 0.73 | 0.580664 |
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Target: 5'- cGAUCG-UUGCGcACCACCGUUCGugUGg -3' miRNA: 3'- -CUAGCaGACGC-UGGUGGCGAGCuuGC- -5' |
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| 12709 | 3' | -54.9 | NC_003349.1 | + | 43893 | 0.74 | 0.520203 |
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Target: 5'- -uUCGUC-GCGGCCACUGCU-GAACa -3' miRNA: 3'- cuAGCAGaCGCUGGUGGCGAgCUUGc -5' |
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| 12709 | 3' | -54.9 | NC_003349.1 | + | 82045 | 0.87 | 0.100488 |
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Target: 5'- ---nGUCUGCGACCACCGCcCGAACGc -3' miRNA: 3'- cuagCAGACGCUGGUGGCGaGCUUGC- -5' |
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| 12709 | 3' | -54.9 | NC_003349.1 | + | 82020 | 1.08 | 0.003747 |
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Target: 5'- aGAUCGUCUGCGACCACCGCUCGAACGu -3' miRNA: 3'- -CUAGCAGACGCUGGUGGCGAGCUUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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