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miRNA: 3'- cgUCCAGUUauagCCGCGCGcUGUAU- -5'
miRNA: 3'- cguCCAGU---UAUAGcCGCGCgCugUAU- -5'
miRNA: 3'- -CGUCCA------GUUAUAG-CCGCG--------CGCUGUau -5'
miRNA: 3'- -CGUCCAGUUauAGCCG-CGCGcUGUau -5'
miRNA: 3'- -CGUCCAGUUAUAGCCGCGCGCUGUAU- -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio#
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P value |
| Predicted miRNA align pattern | |||||||
| 1271 | 3' | -52.3 | NC_001317.1 | + | 9092 | 0.67 | 0.64279 |
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Target: 5'- -gAGGUCGGg---GGCGCGCuGCAUGu -3' miRNA: 3'- cgUCCAGUUauagCCGCGCGcUGUAU- -5' |
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| 1271 | 3' | -52.3 | NC_001317.1 | + | 5323 | 0.67 | 0.630987 |
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Target: 5'- --cGGUCAgagGUGUCaGCGCGgGugGUAc -3' miRNA: 3'- cguCCAGU---UAUAGcCGCGCgCugUAU- -5' |
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| 1271 | 3' | -52.3 | NC_001317.1 | + | 737 | 0.69 | 0.503631 |
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Target: 5'- aGCAGGUgaaacaCGGUAUCaGGCGCgaauucauGCGGCAg- -3' miRNA: 3'- -CGUCCA------GUUAUAG-CCGCG--------CGCUGUau -5' |
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| 1271 | 3' | -52.3 | NC_001317.1 | + | 15491 | 0.7 | 0.449324 |
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Target: 5'- uGCGGGUCAGcuUCGGCaGCGUcACGg- -3' miRNA: 3'- -CGUCCAGUUauAGCCG-CGCGcUGUau -5' |
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| 1271 | 3' | -52.3 | NC_001317.1 | + | 27177 | 1.09 | 0.000829 |
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Target: 5'- aGCAGGUCAAUAUCGGCGCGCGACAUAu -3' miRNA: 3'- -CGUCCAGUUAUAGCCGCGCGCUGUAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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