Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12710 | 3' | -56 | NC_003349.1 | + | 113758 | 0.66 | 0.89526 |
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Target: 5'- uCUGCGUCcuCGACUGuuGCUucGGCUg -3' miRNA: 3'- -GACGCAGcuGCUGGCggCGAucUUGA- -5' |
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| 12710 | 3' | -56 | NC_003349.1 | + | 125402 | 0.66 | 0.88854 |
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Target: 5'- --uUGUCGugGACaUGCCGCU-GAAUUg -3' miRNA: 3'- gacGCAGCugCUG-GCGGCGAuCUUGA- -5' |
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| 12710 | 3' | -56 | NC_003349.1 | + | 82207 | 0.73 | 0.515906 |
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Target: 5'- nUGCGUCGAccgcccgaacgucuaCGGCCGCCGCcgccgccuGAGCg -3' miRNA: 3'- gACGCAGCU---------------GCUGGCGGCGau------CUUGa -5' |
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| 12710 | 3' | -56 | NC_003349.1 | + | 17577 | 0.73 | 0.502242 |
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Target: 5'- uUGUGaacUGACGACUGCCGCUgauaAGAACUc -3' miRNA: 3'- gACGCa--GCUGCUGGCGGCGA----UCUUGA- -5' |
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| 12710 | 3' | -56 | NC_003349.1 | + | 113788 | 0.75 | 0.436553 |
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Target: 5'- uCUGCGUCcGCGACUGuuGCUAcGGCUg -3' miRNA: 3'- -GACGCAGcUGCUGGCggCGAUcUUGA- -5' |
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| 12710 | 3' | -56 | NC_003349.1 | + | 82182 | 1.09 | 0.002753 |
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Target: 5'- aCUGCGUCGACGACCGCCGCUAGAACUg -3' miRNA: 3'- -GACGCAGCUGCUGGCGGCGAUCUUGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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