Results 1 - 13 of 13 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12712 | 3' | -55.4 | NC_003349.1 | + | 82320 | 0.66 | 0.893096 |
Target: 5'- nGACGCAGUUc---CAGCGGUgGCGGu -3' miRNA: 3'- uCUGCGUCGGcaucGUUGUCAgCGCC- -5' |
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12712 | 3' | -55.4 | NC_003349.1 | + | 116618 | 0.66 | 0.886262 |
Target: 5'- cGGugGCGGCUGcAGCAAC-GUUGUu- -3' miRNA: 3'- -UCugCGUCGGCaUCGUUGuCAGCGcc -5' |
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12712 | 3' | -55.4 | NC_003349.1 | + | 82413 | 0.66 | 0.886262 |
Target: 5'- ---aGCAGCaGcGGCAGCGGUgGUGGa -3' miRNA: 3'- ucugCGUCGgCaUCGUUGUCAgCGCC- -5' |
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12712 | 3' | -55.4 | NC_003349.1 | + | 26928 | 0.67 | 0.864405 |
Target: 5'- cGGAU--GGCCG-AGCAGCAGUCacuuGUGGa -3' miRNA: 3'- -UCUGcgUCGGCaUCGUUGUCAG----CGCC- -5' |
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12712 | 3' | -55.4 | NC_003349.1 | + | 82208 | 0.67 | 0.856684 |
Target: 5'- gAGGuCGCAGgCGUucgGGCggUGGUCGCa- -3' miRNA: 3'- -UCU-GCGUCgGCA---UCGuuGUCAGCGcc -5' |
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12712 | 3' | -55.4 | NC_003349.1 | + | 66126 | 0.67 | 0.848757 |
Target: 5'- aAGGCGCGGaaaaCGUgagcGGCGACAG-CGUGu -3' miRNA: 3'- -UCUGCGUCg---GCA----UCGUUGUCaGCGCc -5' |
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12712 | 3' | -55.4 | NC_003349.1 | + | 17141 | 0.68 | 0.797244 |
Target: 5'- cGGugGCAacaCCG--GCGACGGUCGCGu -3' miRNA: 3'- -UCugCGUc--GGCauCGUUGUCAGCGCc -5' |
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12712 | 3' | -55.4 | NC_003349.1 | + | 113460 | 0.7 | 0.697192 |
Target: 5'- uGACGCGgugccaaaccggccGCCGUAGaaacgGGCAGUCGCa- -3' miRNA: 3'- uCUGCGU--------------CGGCAUCg----UUGUCAGCGcc -5' |
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12712 | 3' | -55.4 | NC_003349.1 | + | 1534 | 0.74 | 0.477532 |
Target: 5'- gAGGCgGCGGCgGUGGCGGCGGUauugGUGGu -3' miRNA: 3'- -UCUG-CGUCGgCAUCGUUGUCAg---CGCC- -5' |
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12712 | 3' | -55.4 | NC_003349.1 | + | 113655 | 0.74 | 0.468047 |
Target: 5'- cAGACGCAGCCGaAGCcgUAGcCGCa- -3' miRNA: 3'- -UCUGCGUCGGCaUCGuuGUCaGCGcc -5' |
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12712 | 3' | -55.4 | NC_003349.1 | + | 82344 | 0.76 | 0.379077 |
Target: 5'- cGACGCAGUUcUAGCGGCGGUCGuCGa -3' miRNA: 3'- uCUGCGUCGGcAUCGUUGUCAGC-GCc -5' |
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12712 | 3' | -55.4 | NC_003349.1 | + | 113595 | 0.84 | 0.139759 |
Target: 5'- cAGuCGCAGCCGaAGCAACAGUCGaGGa -3' miRNA: 3'- -UCuGCGUCGGCaUCGUUGUCAGCgCC- -5' |
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12712 | 3' | -55.4 | NC_003349.1 | + | 113625 | 1.11 | 0.002158 |
Target: 5'- cAGACGCAGCCGUAGCAACAGUCGCGGa -3' miRNA: 3'- -UCUGCGUCGGCAUCGUUGUCAGCGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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