Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12712 | 5' | -58.9 | NC_003349.1 | + | 51871 | 0.66 | 0.727315 |
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Target: 5'- uAGCCaaaccGUCGgauCCGCAGCUGUACa -3' miRNA: 3'- -UCGGcuu--CGGCaucGGCGUCGGCAUG- -5' |
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| 12712 | 5' | -58.9 | NC_003349.1 | + | 112185 | 0.67 | 0.687341 |
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Target: 5'- uGUCG-AGuuGa--CCGCGGCCGUACg -3' miRNA: 3'- uCGGCuUCggCaucGGCGUCGGCAUG- -5' |
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| 12712 | 5' | -58.9 | NC_003349.1 | + | 82383 | 0.67 | 0.67721 |
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Target: 5'- cGCUcAGGCgGcGGCgGCGGCCGUAg -3' miRNA: 3'- uCGGcUUCGgCaUCGgCGUCGGCAUg -5' |
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| 12712 | 5' | -58.9 | NC_003349.1 | + | 113547 | 0.69 | 0.575242 |
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Target: 5'- gAGCCGAAGUcgaucucgucgaCGUAGUguCAGCCGUAa -3' miRNA: 3'- -UCGGCUUCG------------GCAUCGgcGUCGGCAUg -5' |
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| 12712 | 5' | -58.9 | NC_003349.1 | + | 66051 | 0.7 | 0.496134 |
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Target: 5'- uAGCCGAAGCUGUAGCCaGUAauacuCCGa-- -3' miRNA: 3'- -UCGGCUUCGGCAUCGG-CGUc----GGCaug -5' |
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| 12712 | 5' | -58.9 | NC_003349.1 | + | 113512 | 0.7 | 0.486572 |
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Target: 5'- cGUCGAAGUCagAGUCGCAGCUGUucACg -3' miRNA: 3'- uCGGCUUCGGcaUCGGCGUCGGCA--UG- -5' |
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| 12712 | 5' | -58.9 | NC_003349.1 | + | 113601 | 0.73 | 0.363429 |
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Target: 5'- cAGCCGAAGCaacaGUcgaggacgcAGaCGCAGCCGUAg -3' miRNA: 3'- -UCGGCUUCGg---CA---------UCgGCGUCGGCAUg -5' |
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| 12712 | 5' | -58.9 | NC_003349.1 | + | 113661 | 1.09 | 0.001208 |
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Target: 5'- cAGCCGAAGCCGUAGCCGCAGCCGUACu -3' miRNA: 3'- -UCGGCUUCGGCAUCGGCGUCGGCAUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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