Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12713 | 5' | -48.7 | NC_003349.1 | + | 83835 | 0.66 | 0.997338 |
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Target: 5'- cGUCGcGAAgauUGuuGUCGACGuuGACg -3' miRNA: 3'- aCAGCaCUU---ACggCGGCUGUuuUUGa -5' |
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| 12713 | 5' | -48.7 | NC_003349.1 | + | 86205 | 0.66 | 0.997338 |
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Target: 5'- -aUCGUcGAA--CCGCCGACGAuuGCa -3' miRNA: 3'- acAGCA-CUUacGGCGGCUGUUuuUGa -5' |
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| 12713 | 5' | -48.7 | NC_003349.1 | + | 123780 | 0.69 | 0.982278 |
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Target: 5'- -aUCGUG-AUGUCGCCGAUcuGGAUa -3' miRNA: 3'- acAGCACuUACGGCGGCUGuuUUUGa -5' |
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| 12713 | 5' | -48.7 | NC_003349.1 | + | 87239 | 0.72 | 0.924638 |
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Target: 5'- gGUCGUGuacGUGCCaaCCGACGAcGACa -3' miRNA: 3'- aCAGCACu--UACGGc-GGCUGUUuUUGa -5' |
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| 12713 | 5' | -48.7 | NC_003349.1 | + | 125402 | 0.73 | 0.88516 |
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Target: 5'- uUGUCGUGGAcaUGCCGCUGAauuGuuACg -3' miRNA: 3'- -ACAGCACUU--ACGGCGGCUgu-UuuUGa -5' |
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| 12713 | 5' | -48.7 | NC_003349.1 | + | 77327 | 0.74 | 0.869926 |
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Target: 5'- cGUCGUcAccGCCGCUGACAAuuauGACUa -3' miRNA: 3'- aCAGCAcUuaCGGCGGCUGUUu---UUGA- -5' |
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| 12713 | 5' | -48.7 | NC_003349.1 | + | 71880 | 0.75 | 0.818653 |
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Target: 5'- aGUCGUGuaguUGCCGUCGGCAcgguAACa -3' miRNA: 3'- aCAGCACuu--ACGGCGGCUGUuu--UUGa -5' |
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| 12713 | 5' | -48.7 | NC_003349.1 | + | 17579 | 0.83 | 0.40649 |
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Target: 5'- aGUUGUGAAcugacgacUGCCGCUGAUAAGAACUc -3' miRNA: 3'- aCAGCACUU--------ACGGCGGCUGUUUUUGA- -5' |
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| 12713 | 5' | -48.7 | NC_003349.1 | + | 120523 | 1.08 | 0.01393 |
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Target: 5'- cUGUCGUGAAUGCCGCCGACAAAAACUc -3' miRNA: 3'- -ACAGCACUUACGGCGGCUGUUUUUGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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