Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12719 | 5' | -54.9 | NC_003356.1 | + | 19228 | 0.66 | 0.661947 |
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Target: 5'- uGUuuGGUG-CGu---UCGCCGGGGCGu -3' miRNA: 3'- -CGggCCAUaGUcauuAGUGGCCCCGU- -5' |
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| 12719 | 5' | -54.9 | NC_003356.1 | + | 36683 | 0.67 | 0.605855 |
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Target: 5'- gGCCuaCGGU-UCAGaaUGAUCGCaCGGGGUc -3' miRNA: 3'- -CGG--GCCAuAGUC--AUUAGUG-GCCCCGu -5' |
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| 12719 | 5' | -54.9 | NC_003356.1 | + | 39542 | 0.67 | 0.605855 |
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Target: 5'- cGCCCGGUG-CAGguucUGAUUAauGGGGUc -3' miRNA: 3'- -CGGGCCAUaGUC----AUUAGUggCCCCGu -5' |
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| 12719 | 5' | -54.9 | NC_003356.1 | + | 36307 | 0.68 | 0.550334 |
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Target: 5'- uUCCGGUAUUguGGUGA--ACgGGGGCGg -3' miRNA: 3'- cGGGCCAUAG--UCAUUagUGgCCCCGU- -5' |
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| 12719 | 5' | -54.9 | NC_003356.1 | + | 21258 | 0.68 | 0.517759 |
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Target: 5'- uGUCCGGUAUuugcCGGUAugaaAUUACCGGaaGGCGc -3' miRNA: 3'- -CGGGCCAUA----GUCAU----UAGUGGCC--CCGU- -5' |
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| 12719 | 5' | -54.9 | NC_003356.1 | + | 24124 | 0.69 | 0.485979 |
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Target: 5'- cCCCGGcugGUUuuGUuGUUGCCGGGGCGc -3' miRNA: 3'- cGGGCCa--UAGu-CAuUAGUGGCCCCGU- -5' |
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| 12719 | 5' | -54.9 | NC_003356.1 | + | 41581 | 0.71 | 0.387636 |
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Target: 5'- gGCCCGGaUGUUaAGUuuUauaACCGGGGCu -3' miRNA: 3'- -CGGGCC-AUAG-UCAuuAg--UGGCCCCGu -5' |
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| 12719 | 5' | -54.9 | NC_003356.1 | + | 12423 | 1.11 | 0.000559 |
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Target: 5'- cGCCCGGUAUCAGUAAUCACCGGGGCAa -3' miRNA: 3'- -CGGGCCAUAGUCAUUAGUGGCCCCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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