Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12720 | 3' | -51.7 | NC_003356.1 | + | 37306 | 0.66 | 0.856059 |
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Target: 5'- -aCGGUGAccGCAGCcgauggucuucuCCGggUGCCuGUUGu -3' miRNA: 3'- agGCCAUU--UGUCG------------GGCuuAUGG-CAGC- -5' |
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| 12720 | 3' | -51.7 | NC_003356.1 | + | 34968 | 0.66 | 0.847301 |
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Target: 5'- uUCCGGUcgcgaaugGGGCGGCUgGAcauCCGUCc -3' miRNA: 3'- -AGGCCA--------UUUGUCGGgCUuauGGCAGc -5' |
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| 12720 | 3' | -51.7 | NC_003356.1 | + | 12877 | 0.66 | 0.829072 |
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Target: 5'- gCCGGUAu-CAcGCCUGAAauCUGUCGc -3' miRNA: 3'- aGGCCAUuuGU-CGGGCUUauGGCAGC- -5' |
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| 12720 | 3' | -51.7 | NC_003356.1 | + | 24681 | 0.67 | 0.809956 |
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Target: 5'- cUUCGGUuccacACAGCCCGGAU-CUaUCGg -3' miRNA: 3'- -AGGCCAuu---UGUCGGGCUUAuGGcAGC- -5' |
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| 12720 | 3' | -51.7 | NC_003356.1 | + | 22858 | 0.69 | 0.67062 |
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Target: 5'- gUCCGGaGGACGguGCCCGuuuugaACCGUUGa -3' miRNA: 3'- -AGGCCaUUUGU--CGGGCuua---UGGCAGC- -5' |
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| 12720 | 3' | -51.7 | NC_003356.1 | + | 35122 | 0.7 | 0.659283 |
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Target: 5'- aUCGGUGAugAGCCCGugcucACCGg-- -3' miRNA: 3'- aGGCCAUUugUCGGGCuua--UGGCagc -5' |
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| 12720 | 3' | -51.7 | NC_003356.1 | + | 29471 | 0.73 | 0.481212 |
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Target: 5'- --aGGUuAACAGCCUGGugcugAUGCCGUCa -3' miRNA: 3'- aggCCAuUUGUCGGGCU-----UAUGGCAGc -5' |
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| 12720 | 3' | -51.7 | NC_003356.1 | + | 24371 | 0.73 | 0.450134 |
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Target: 5'- uUCCGGUAuaggugucguauGACAGCCCGAuaGCC-UCc -3' miRNA: 3'- -AGGCCAU------------UUGUCGGGCUuaUGGcAGc -5' |
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| 12720 | 3' | -51.7 | NC_003356.1 | + | 19254 | 1.09 | 0.001891 |
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Target: 5'- uUCCGGUAAACAGCCCGAAUACCGUCGc -3' miRNA: 3'- -AGGCCAUUUGUCGGGCUUAUGGCAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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