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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 12720 | 5' | -63.1 | NC_003356.1 | + | 37307 | 0.67 | 0.228112 |
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Target: 5'- cGGUGaCCGCAGCCGauggucuucuccgggUGCCuguuguggcagacGAACCGGGa -3' miRNA: 3'- -UCGC-GGCGUCGGC---------------ACGGc------------CUUGGCCU- -5' |
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| 12720 | 5' | -63.1 | NC_003356.1 | + | 35331 | 0.66 | 0.25867 |
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Target: 5'- cGUGCCGCagacAGCgGUgauugaugcccaGCCGGAACaCGGc -3' miRNA: 3'- uCGCGGCG----UCGgCA------------CGGCCUUG-GCCu -5' |
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| 12720 | 5' | -63.1 | NC_003356.1 | + | 31712 | 0.69 | 0.162727 |
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Target: 5'- gGGCGCaGCGG--GUGCCGGAACCGc- -3' miRNA: 3'- -UCGCGgCGUCggCACGGCCUUGGCcu -5' |
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| 12720 | 5' | -63.1 | NC_003356.1 | + | 28064 | 0.66 | 0.295342 |
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Target: 5'- -aCGCCGCuGaCCGUucaggucgcguauucGCCGGAuCCGGu -3' miRNA: 3'- ucGCGGCGuC-GGCA---------------CGGCCUuGGCCu -5' |
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| 12720 | 5' | -63.1 | NC_003356.1 | + | 27993 | 0.71 | 0.114701 |
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Target: 5'- -cUGCCGUgauGUCGUGCCGGAAaCGGAu -3' miRNA: 3'- ucGCGGCGu--CGGCACGGCCUUgGCCU- -5' |
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| 12720 | 5' | -63.1 | NC_003356.1 | + | 19220 | 1.08 | 0.000159 |
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Target: 5'- gAGCGCCGCAGCCGUGCCGGAACCGGAc -3' miRNA: 3'- -UCGCGGCGUCGGCACGGCCUUGGCCU- -5' |
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| 12720 | 5' | -63.1 | NC_003356.1 | + | 17905 | 0.66 | 0.292492 |
|
Target: 5'- cGUGUCGCAGC---GCUGGAAcguuCCGGAa -3' miRNA: 3'- uCGCGGCGUCGgcaCGGCCUU----GGCCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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