Results 1 - 19 of 19 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12721 | 3' | -55.6 | NC_003356.1 | + | 37691 | 0.66 | 0.618151 |
Target: 5'- uUCAGGAucguuUGuCGCCaCCAuCACGCCAa -3' miRNA: 3'- uGGUCUUuu---AC-GCGGcGGU-GUGCGGU- -5' |
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12721 | 3' | -55.6 | NC_003356.1 | + | 20738 | 0.66 | 0.606849 |
Target: 5'- uCCuGGugGGAUGCGUCaGCCuggauaaaGCGCGCCAg -3' miRNA: 3'- uGGuCU--UUUACGCGG-CGG--------UGUGCGGU- -5' |
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12721 | 3' | -55.6 | NC_003356.1 | + | 17972 | 0.66 | 0.606849 |
Target: 5'- uCCGGAAcGUuccaGCGCUGCgACACGUUg -3' miRNA: 3'- uGGUCUUuUA----CGCGGCGgUGUGCGGu -5' |
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12721 | 3' | -55.6 | NC_003356.1 | + | 24727 | 0.66 | 0.603463 |
Target: 5'- cAUCAGGAAAcGUcaccugauaaaccgGCUGCCACugGCUg -3' miRNA: 3'- -UGGUCUUUUaCG--------------CGGCGGUGugCGGu -5' |
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12721 | 3' | -55.6 | NC_003356.1 | + | 40694 | 0.66 | 0.595571 |
Target: 5'- gUCAGGcaGGAUGCugaaGCCGCCaaACAgGCCGc -3' miRNA: 3'- uGGUCU--UUUACG----CGGCGG--UGUgCGGU- -5' |
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12721 | 3' | -55.6 | NC_003356.1 | + | 7667 | 0.66 | 0.584324 |
Target: 5'- cGCCAGucauUGCGaagauuUGCCACAUGCUg -3' miRNA: 3'- -UGGUCuuuuACGCg-----GCGGUGUGCGGu -5' |
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12721 | 3' | -55.6 | NC_003356.1 | + | 28694 | 0.67 | 0.550875 |
Target: 5'- uGCCGGAAAAcUGCGaCUGCCuGC-UGCCu -3' miRNA: 3'- -UGGUCUUUU-ACGC-GGCGG-UGuGCGGu -5' |
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12721 | 3' | -55.6 | NC_003356.1 | + | 24173 | 0.67 | 0.5289 |
Target: 5'- aGCCGGggGAUaaaucaGCCaggcaCCAUACGCCAg -3' miRNA: 3'- -UGGUCuuUUAcg----CGGc----GGUGUGCGGU- -5' |
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12721 | 3' | -55.6 | NC_003356.1 | + | 26868 | 0.67 | 0.527809 |
Target: 5'- aGCCAGAAAAccuuuugguuuuuUGCugccGUCGCCACugGUa- -3' miRNA: 3'- -UGGUCUUUU-------------ACG----CGGCGGUGugCGgu -5' |
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12721 | 3' | -55.6 | NC_003356.1 | + | 12161 | 0.68 | 0.475529 |
Target: 5'- gACCuGGAAG-GCGCUGUCAUugGCg- -3' miRNA: 3'- -UGGuCUUUUaCGCGGCGGUGugCGgu -5' |
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12721 | 3' | -55.6 | NC_003356.1 | + | 39533 | 0.68 | 0.475529 |
Target: 5'- gACCuGAugGUGCauucCCGCCACGC-CCAa -3' miRNA: 3'- -UGGuCUuuUACGc---GGCGGUGUGcGGU- -5' |
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12721 | 3' | -55.6 | NC_003356.1 | + | 11481 | 0.68 | 0.444821 |
Target: 5'- cACCAGAAuAUGCGCaUGgUAUuCGCCAu -3' miRNA: 3'- -UGGUCUUuUACGCG-GCgGUGuGCGGU- -5' |
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12721 | 3' | -55.6 | NC_003356.1 | + | 19967 | 0.69 | 0.405673 |
Target: 5'- aGCCAGAccAUGaacaGCCGCCACcUGaCCGg -3' miRNA: 3'- -UGGUCUuuUACg---CGGCGGUGuGC-GGU- -5' |
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12721 | 3' | -55.6 | NC_003356.1 | + | 11628 | 0.7 | 0.377773 |
Target: 5'- aGCCAGAAAucuUGCggccugucGCCGCUaugcggagaACugGCCAg -3' miRNA: 3'- -UGGUCUUUu--ACG--------CGGCGG---------UGugCGGU- -5' |
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12721 | 3' | -55.6 | NC_003356.1 | + | 35543 | 0.71 | 0.334224 |
Target: 5'- cCCAGAAccaGCGCCGUCGC-CGCUu -3' miRNA: 3'- uGGUCUUuuaCGCGGCGGUGuGCGGu -5' |
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12721 | 3' | -55.6 | NC_003356.1 | + | 2992 | 0.71 | 0.325967 |
Target: 5'- cGCCAGAAGAU-CGCCGaCGCACucaaccgacugGCCAg -3' miRNA: 3'- -UGGUCUUUUAcGCGGCgGUGUG-----------CGGU- -5' |
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12721 | 3' | -55.6 | NC_003356.1 | + | 22899 | 0.71 | 0.325967 |
Target: 5'- cCCGGAGAcgGCGCguCGCCGaguuGCGCCc -3' miRNA: 3'- uGGUCUUUuaCGCG--GCGGUg---UGCGGu -5' |
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12721 | 3' | -55.6 | NC_003356.1 | + | 19394 | 0.73 | 0.251741 |
Target: 5'- uGCCAGAAccucUGCGCC-CaGCACGCCGa -3' miRNA: 3'- -UGGUCUUuu--ACGCGGcGgUGUGCGGU- -5' |
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12721 | 3' | -55.6 | NC_003356.1 | + | 19355 | 1.08 | 0.000636 |
Target: 5'- cACCAGAAAAUGCGCCGCCACACGCCAg -3' miRNA: 3'- -UGGUCUUUUACGCGGCGGUGUGCGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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