Results 21 - 23 of 23 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12721 | 5' | -46.9 | NC_003356.1 | + | 11195 | 0.72 | 0.770387 |
Target: 5'- aGCGGCuggcUGUUGCCAgAAaucuucgaaGUGACGa -3' miRNA: 3'- aCGCCGua--ACAACGGUgUU---------UAUUGCg -5' |
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12721 | 5' | -46.9 | NC_003356.1 | + | 5735 | 0.68 | 0.92928 |
Target: 5'- gGCGGU--UGUUaaGCCGCAGGcuuCGCg -3' miRNA: 3'- aCGCCGuaACAA--CGGUGUUUauuGCG- -5' |
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12721 | 5' | -46.9 | NC_003356.1 | + | 2237 | 0.67 | 0.959728 |
Target: 5'- --aGGCAgcGUUGCgUACAggcuuucAAUAGCGCa -3' miRNA: 3'- acgCCGUaaCAACG-GUGU-------UUAUUGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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