Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12722 | 3' | -50.8 | NC_003356.1 | + | 27128 | 0.66 | 0.870488 |
Target: 5'- cCAUaCCCuGCCAGaGAAGAAGGCugacCCAg -3' miRNA: 3'- cGUA-GGG-UGGUCcUUUUUUCCGu---GGU- -5' |
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12722 | 3' | -50.8 | NC_003356.1 | + | 26001 | 0.69 | 0.71918 |
Target: 5'- aGCAUUCaugGCCGGGggGuauGGCGCUu -3' miRNA: 3'- -CGUAGGg--UGGUCCuuUuuuCCGUGGu -5' |
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12722 | 3' | -50.8 | NC_003356.1 | + | 28217 | 0.69 | 0.71918 |
Target: 5'- gGCGUUCUggcCCGGGAGAGAacccgGGGUAUCAg -3' miRNA: 3'- -CGUAGGGu--GGUCCUUUUU-----UCCGUGGU- -5' |
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12722 | 3' | -50.8 | NC_003356.1 | + | 19490 | 0.7 | 0.661678 |
Target: 5'- --cUCCCGCCugcGGGGugGAGGAGGCGgCAa -3' miRNA: 3'- cguAGGGUGG---UCCU--UUUUUCCGUgGU- -5' |
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12722 | 3' | -50.8 | NC_003356.1 | + | 36235 | 0.71 | 0.557178 |
Target: 5'- aUcgCCCGCCGGGAAuAAAGcaacgcccucaGCACCGu -3' miRNA: 3'- cGuaGGGUGGUCCUUuUUUC-----------CGUGGU- -5' |
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12722 | 3' | -50.8 | NC_003356.1 | + | 20793 | 1.12 | 0.001145 |
Target: 5'- cGCAUCCCACCAGGAAAAAAGGCACCAg -3' miRNA: 3'- -CGUAGGGUGGUCCUUUUUUCCGUGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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