miRNA display CGI


Results 1 - 8 of 8 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
12723 5' -47.6 NC_003356.1 + 39246 0.66 0.974517
Target:  5'- cCGAUAcccagCGCAccauuGACCUGAGAAccGCc -3'
miRNA:   3'- aGCUGUuaaa-GCGU-----CUGGACUCUU--CG- -5'
12723 5' -47.6 NC_003356.1 + 31820 0.66 0.967974
Target:  5'- -gGGCAAUg--GCAGAUUUGAccgGGAGCg -3'
miRNA:   3'- agCUGUUAaagCGUCUGGACU---CUUCG- -5'
12723 5' -47.6 NC_003356.1 + 20855 1.14 0.002252
Target:  5'- gUCGACAAUUUCGCAGACCUGAGAAGCu -3'
miRNA:   3'- -AGCUGUUAAAGCGUCUGGACUCUUCG- -5'
12723 5' -47.6 NC_003356.1 + 20819 0.66 0.967974
Target:  5'- uUCGGCAGUgUCuGCAGaacugaucGCCUGuaaGAGCa -3'
miRNA:   3'- -AGCUGUUAaAG-CGUC--------UGGACuc-UUCG- -5'
12723 5' -47.6 NC_003356.1 + 12487 0.69 0.888522
Target:  5'- aCGugAug--CGCAuACCUGuGAAGCa -3'
miRNA:   3'- aGCugUuaaaGCGUcUGGACuCUUCG- -5'
12723 5' -47.6 NC_003356.1 + 10498 0.68 0.935924
Target:  5'- aCGGCAAUUUC--AGAgCUG-GAGGCu -3'
miRNA:   3'- aGCUGUUAAAGcgUCUgGACuCUUCG- -5'
12723 5' -47.6 NC_003356.1 + 3864 0.68 0.935924
Target:  5'- uUCGACAugGUUUUGCAGGgCaaUGcGAAGUg -3'
miRNA:   3'- -AGCUGU--UAAAGCGUCUgG--ACuCUUCG- -5'
12723 5' -47.6 NC_003356.1 + 1793 0.67 0.951487
Target:  5'- aCGGCAA--UCGCuauGACCUGAc--GCg -3'
miRNA:   3'- aGCUGUUaaAGCGu--CUGGACUcuuCG- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.