Results 1 - 20 of 22 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12724 | 5' | -56.6 | NC_003356.1 | + | 13257 | 0.66 | 0.560055 |
Target: 5'- gGGCCugGACUccacaccgcgggAGAGCuGaCuCAGCGCa -3' miRNA: 3'- -UCGGugCUGG------------UCUUGuCcGuGUCGCG- -5' |
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12724 | 5' | -56.6 | NC_003356.1 | + | 32000 | 0.66 | 0.558956 |
Target: 5'- -aCCACGGCggaagaauccgguCAGAugAuGGCACaguGGCGCa -3' miRNA: 3'- ucGGUGCUG-------------GUCUugU-CCGUG---UCGCG- -5' |
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12724 | 5' | -56.6 | NC_003356.1 | + | 19073 | 0.66 | 0.549098 |
Target: 5'- cAGUagaGGCCAGAGCAcGGC-CAGCa- -3' miRNA: 3'- -UCGgugCUGGUCUUGU-CCGuGUCGcg -5' |
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12724 | 5' | -56.6 | NC_003356.1 | + | 41737 | 0.66 | 0.548006 |
Target: 5'- cAGCCACG-CCAGAcgauacagcgaaaACcGGC-CAGCa- -3' miRNA: 3'- -UCGGUGCuGGUCU-------------UGuCCGuGUCGcg -5' |
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12724 | 5' | -56.6 | NC_003356.1 | + | 34889 | 0.66 | 0.538209 |
Target: 5'- -aCCAUGacgcaGCCAGAACAaGCGCAG-GCa -3' miRNA: 3'- ucGGUGC-----UGGUCUUGUcCGUGUCgCG- -5' |
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12724 | 5' | -56.6 | NC_003356.1 | + | 30015 | 0.66 | 0.527395 |
Target: 5'- cAGCgGCGGuaAGAuCAGGCGCGcCGCu -3' miRNA: 3'- -UCGgUGCUggUCUuGUCCGUGUcGCG- -5' |
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12724 | 5' | -56.6 | NC_003356.1 | + | 9591 | 0.66 | 0.516664 |
Target: 5'- cGCgCACG-CCAGAAUuaugguGGGCugGGCa- -3' miRNA: 3'- uCG-GUGCuGGUCUUG------UCCGugUCGcg -5' |
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12724 | 5' | -56.6 | NC_003356.1 | + | 35450 | 0.67 | 0.506021 |
Target: 5'- uGGuUCugGGaaaacCCGGAACAGGUaaagcggcgACGGCGCu -3' miRNA: 3'- -UC-GGugCU-----GGUCUUGUCCG---------UGUCGCG- -5' |
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12724 | 5' | -56.6 | NC_003356.1 | + | 27990 | 0.67 | 0.506021 |
Target: 5'- cGGCgaauaCGCGACCuGAACGGucaGCGGCGUu -3' miRNA: 3'- -UCG-----GUGCUGGuCUUGUCcg-UGUCGCG- -5' |
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12724 | 5' | -56.6 | NC_003356.1 | + | 12578 | 0.67 | 0.502846 |
Target: 5'- cAGCCuguacauauugaugAUGugcuuCCGGAGguGGUugAGCGCa -3' miRNA: 3'- -UCGG--------------UGCu----GGUCUUguCCGugUCGCG- -5' |
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12724 | 5' | -56.6 | NC_003356.1 | + | 38555 | 0.67 | 0.495473 |
Target: 5'- -cCCACGuCCGGAucgguguguuGCAGGCGuCuGUGCa -3' miRNA: 3'- ucGGUGCuGGUCU----------UGUCCGU-GuCGCG- -5' |
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12724 | 5' | -56.6 | NC_003356.1 | + | 16338 | 0.67 | 0.495473 |
Target: 5'- aGGCgAUGACCacAGAACAGGUaaagacuuccgGCAG-GCu -3' miRNA: 3'- -UCGgUGCUGG--UCUUGUCCG-----------UGUCgCG- -5' |
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12724 | 5' | -56.6 | NC_003356.1 | + | 31712 | 0.67 | 0.474684 |
Target: 5'- gGGCgcaGCGgguGCCGGAACcgcaguacuggGGGCGCuGGCGCu -3' miRNA: 3'- -UCGg--UGC---UGGUCUUG-----------UCCGUG-UCGCG- -5' |
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12724 | 5' | -56.6 | NC_003356.1 | + | 2787 | 0.68 | 0.440386 |
Target: 5'- aAGUCACGgcgaACCAGAaaacacuGCAGcGCgagcaggaaguaaaACAGCGCg -3' miRNA: 3'- -UCGGUGC----UGGUCU-------UGUC-CG--------------UGUCGCG- -5' |
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12724 | 5' | -56.6 | NC_003356.1 | + | 20001 | 0.68 | 0.42474 |
Target: 5'- -uCCGCGACCAGuuguuuCuGGCGCucuuucguGCGCa -3' miRNA: 3'- ucGGUGCUGGUCuu----GuCCGUGu-------CGCG- -5' |
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12724 | 5' | -56.6 | NC_003356.1 | + | 11146 | 0.68 | 0.405658 |
Target: 5'- cAGCCGCucCCGGAGguGGgCgACAGCGa -3' miRNA: 3'- -UCGGUGcuGGUCUUguCC-G-UGUCGCg -5' |
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12724 | 5' | -56.6 | NC_003356.1 | + | 34343 | 0.68 | 0.405658 |
Target: 5'- --gCugGAuCCAGGACuGGCACcGCGUg -3' miRNA: 3'- ucgGugCU-GGUCUUGuCCGUGuCGCG- -5' |
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12724 | 5' | -56.6 | NC_003356.1 | + | 7668 | 0.69 | 0.387123 |
Target: 5'- uGGCUAUGuucuCCAucACAGGCAuCAGCGa -3' miRNA: 3'- -UCGGUGCu---GGUcuUGUCCGU-GUCGCg -5' |
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12724 | 5' | -56.6 | NC_003356.1 | + | 41877 | 0.69 | 0.378068 |
Target: 5'- gAGgCAUGGCguaCAGAACAGGa--AGCGCa -3' miRNA: 3'- -UCgGUGCUG---GUCUUGUCCgugUCGCG- -5' |
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12724 | 5' | -56.6 | NC_003356.1 | + | 29922 | 0.69 | 0.360389 |
Target: 5'- cGUgACGgaaGCCGGAACucGGGUAgCGGCGCg -3' miRNA: 3'- uCGgUGC---UGGUCUUG--UCCGU-GUCGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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