Results 1 - 20 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12725 | 5' | -51.5 | NC_003356.1 | + | 24453 | 1.14 | 0.000737 |
Target: 5'- aGCAUACCAACAGACUCAGCCAGCACCc -3' miRNA: 3'- -CGUAUGGUUGUCUGAGUCGGUCGUGG- -5' |
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12725 | 5' | -51.5 | NC_003356.1 | + | 21473 | 0.75 | 0.353393 |
Target: 5'- uGCAgcACCAgcGCGGACUgAGCCGcCACCa -3' miRNA: 3'- -CGUa-UGGU--UGUCUGAgUCGGUcGUGG- -5' |
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12725 | 5' | -51.5 | NC_003356.1 | + | 19075 | 0.74 | 0.38069 |
Target: 5'- cGCAguagagGCCAgaGCAcGGC-CAGCaCAGCACCa -3' miRNA: 3'- -CGUa-----UGGU--UGU-CUGaGUCG-GUCGUGG- -5' |
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12725 | 5' | -51.5 | NC_003356.1 | + | 24173 | 0.74 | 0.399654 |
Target: 5'- ---aGCCGGgGGAUaaaUCAGCCAGgCACCa -3' miRNA: 3'- cguaUGGUUgUCUG---AGUCGGUC-GUGG- -5' |
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12725 | 5' | -51.5 | NC_003356.1 | + | 24319 | 0.74 | 0.380689 |
Target: 5'- cUAUACCGgaaaGCGGAUcagCAGCCAGCggGCCa -3' miRNA: 3'- cGUAUGGU----UGUCUGa--GUCGGUCG--UGG- -5' |
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12725 | 5' | -51.5 | NC_003356.1 | + | 38090 | 0.71 | 0.547259 |
Target: 5'- -gAUACCGACGGugUacuugaCGGUCuGGCACCa -3' miRNA: 3'- cgUAUGGUUGUCugA------GUCGG-UCGUGG- -5' |
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12725 | 5' | -51.5 | NC_003356.1 | + | 19779 | 0.71 | 0.524855 |
Target: 5'- cGCAgaaCAGCAGAUUaaaaaacacuucCAGCCAGC-CCg -3' miRNA: 3'- -CGUaugGUUGUCUGA------------GUCGGUCGuGG- -5' |
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12725 | 5' | -51.5 | NC_003356.1 | + | 22172 | 0.71 | 0.536016 |
Target: 5'- cGCAUAaCCuccuGCugcGACUCAuGCCAGUugCg -3' miRNA: 3'- -CGUAU-GGu---UGu--CUGAGU-CGGUCGugG- -5' |
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12725 | 5' | -51.5 | NC_003356.1 | + | 19041 | 0.7 | 0.604398 |
Target: 5'- ---cACCAACAcgccCUCAGCaAGCACCg -3' miRNA: 3'- cguaUGGUUGUcu--GAGUCGgUCGUGG- -5' |
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12725 | 5' | -51.5 | NC_003356.1 | + | 21729 | 0.7 | 0.627508 |
Target: 5'- gGCAggGCCAgcACAGACUCuGCaGGUugCu -3' miRNA: 3'- -CGUa-UGGU--UGUCUGAGuCGgUCGugG- -5' |
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12725 | 5' | -51.5 | NC_003356.1 | + | 36846 | 0.7 | 0.581394 |
Target: 5'- ---gACCGggACAGACUC-GCUGGCACUu -3' miRNA: 3'- cguaUGGU--UGUCUGAGuCGGUCGUGG- -5' |
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12725 | 5' | -51.5 | NC_003356.1 | + | 39340 | 0.7 | 0.597483 |
Target: 5'- gGCAUAaacauCCAGCAGGCcgucaccauucuguuUCAgGCCGGUAUCg -3' miRNA: 3'- -CGUAU-----GGUUGUCUG---------------AGU-CGGUCGUGG- -5' |
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12725 | 5' | -51.5 | NC_003356.1 | + | 26373 | 0.68 | 0.707906 |
Target: 5'- uGCGUGCCGccugcCGGACgcuugacuUCAGCCAGUucaGCUu -3' miRNA: 3'- -CGUAUGGUu----GUCUG--------AGUCGGUCG---UGG- -5' |
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12725 | 5' | -51.5 | NC_003356.1 | + | 27846 | 0.68 | 0.741387 |
Target: 5'- gGCAa---AGguGGCUCAGCCAGgCGCUg -3' miRNA: 3'- -CGUauggUUguCUGAGUCGGUC-GUGG- -5' |
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12725 | 5' | -51.5 | NC_003356.1 | + | 34798 | 0.68 | 0.730331 |
Target: 5'- aGCGaacGCCAGCGGAagUUCuGCCuGCGCUu -3' miRNA: 3'- -CGUa--UGGUUGUCU--GAGuCGGuCGUGG- -5' |
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12725 | 5' | -51.5 | NC_003356.1 | + | 1634 | 0.68 | 0.719166 |
Target: 5'- uGCAuUACCucACAcaacACUCAGCCcacggcaguGGCACCa -3' miRNA: 3'- -CGU-AUGGu-UGUc---UGAGUCGG---------UCGUGG- -5' |
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12725 | 5' | -51.5 | NC_003356.1 | + | 15710 | 0.67 | 0.784261 |
Target: 5'- aCAUACCAAUA-ACUCuugucgaaaaAGCCAGCAa- -3' miRNA: 3'- cGUAUGGUUGUcUGAG----------UCGGUCGUgg -5' |
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12725 | 5' | -51.5 | NC_003356.1 | + | 2048 | 0.67 | 0.763121 |
Target: 5'- uGUAUACCGACAGu----GCCGGuUACCc -3' miRNA: 3'- -CGUAUGGUUGUCugaguCGGUC-GUGG- -5' |
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12725 | 5' | -51.5 | NC_003356.1 | + | 21635 | 0.67 | 0.752321 |
Target: 5'- cGCAUGgCGgaACAGACgCAGaCAGCaACCu -3' miRNA: 3'- -CGUAUgGU--UGUCUGaGUCgGUCG-UGG- -5' |
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12725 | 5' | -51.5 | NC_003356.1 | + | 25609 | 0.66 | 0.833839 |
Target: 5'- gGCGcUGCCuGCGuuGCUCuGgCAGCACCg -3' miRNA: 3'- -CGU-AUGGuUGUc-UGAGuCgGUCGUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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