miRNA display CGI


Results 1 - 7 of 7 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
12727 5' -48.3 NC_003356.1 + 10928 0.66 0.94044
Target:  5'- aGAACGCGcUGGUgcagacGCGUCaGCGg -3'
miRNA:   3'- gUUUGCGUuACCGaaaa--CGUAGcCGC- -5'
12727 5' -48.3 NC_003356.1 + 31063 0.66 0.934846
Target:  5'- gCAAACGgcAUGGUguaugUGCuuUCGGCGg -3'
miRNA:   3'- -GUUUGCguUACCGaaa--ACGu-AGCCGC- -5'
12727 5' -48.3 NC_003356.1 + 16993 0.67 0.904957
Target:  5'- aAAGCaGCGAUGGCgcugggacgucgUGCaAUUGGCGu -3'
miRNA:   3'- gUUUG-CGUUACCGaaa---------ACG-UAGCCGC- -5'
12727 5' -48.3 NC_003356.1 + 2915 0.69 0.833622
Target:  5'- -uGGC-CAGUcGGUUgagUGCGUCGGCGa -3'
miRNA:   3'- guUUGcGUUA-CCGAaa-ACGUAGCCGC- -5'
12727 5' -48.3 NC_003356.1 + 22859 0.69 0.833622
Target:  5'- --cACGCAAUGGUUccgUGCGUUGcGCc -3'
miRNA:   3'- guuUGCGUUACCGAaa-ACGUAGC-CGc -5'
12727 5' -48.3 NC_003356.1 + 26909 0.71 0.760714
Target:  5'- cCAAACGCAcgGGuCUUgucaucuuCAUCGGUGg -3'
miRNA:   3'- -GUUUGCGUuaCC-GAAaac-----GUAGCCGC- -5'
12727 5' -48.3 NC_003356.1 + 27188 1.11 0.002603
Target:  5'- cCAAACGCAAUGGCUUUUGCAUCGGCGg -3'
miRNA:   3'- -GUUUGCGUUACCGAAAACGUAGCCGC- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.