Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12727 | 5' | -48.3 | NC_003356.1 | + | 10928 | 0.66 | 0.94044 |
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Target: 5'- aGAACGCGcUGGUgcagacGCGUCaGCGg -3' miRNA: 3'- gUUUGCGUuACCGaaaa--CGUAGcCGC- -5' |
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| 12727 | 5' | -48.3 | NC_003356.1 | + | 31063 | 0.66 | 0.934846 |
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Target: 5'- gCAAACGgcAUGGUguaugUGCuuUCGGCGg -3' miRNA: 3'- -GUUUGCguUACCGaaa--ACGu-AGCCGC- -5' |
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| 12727 | 5' | -48.3 | NC_003356.1 | + | 16993 | 0.67 | 0.904957 |
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Target: 5'- aAAGCaGCGAUGGCgcugggacgucgUGCaAUUGGCGu -3' miRNA: 3'- gUUUG-CGUUACCGaaa---------ACG-UAGCCGC- -5' |
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| 12727 | 5' | -48.3 | NC_003356.1 | + | 2915 | 0.69 | 0.833622 |
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Target: 5'- -uGGC-CAGUcGGUUgagUGCGUCGGCGa -3' miRNA: 3'- guUUGcGUUA-CCGAaa-ACGUAGCCGC- -5' |
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| 12727 | 5' | -48.3 | NC_003356.1 | + | 22859 | 0.69 | 0.833622 |
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Target: 5'- --cACGCAAUGGUUccgUGCGUUGcGCc -3' miRNA: 3'- guuUGCGUUACCGAaa-ACGUAGC-CGc -5' |
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| 12727 | 5' | -48.3 | NC_003356.1 | + | 26909 | 0.71 | 0.760714 |
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Target: 5'- cCAAACGCAcgGGuCUUgucaucuuCAUCGGUGg -3' miRNA: 3'- -GUUUGCGUuaCC-GAAaac-----GUAGCCGC- -5' |
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| 12727 | 5' | -48.3 | NC_003356.1 | + | 27188 | 1.11 | 0.002603 |
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Target: 5'- cCAAACGCAAUGGCUUUUGCAUCGGCGg -3' miRNA: 3'- -GUUUGCGUUACCGAAAACGUAGCCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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