Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12728 | 5' | -53.4 | NC_003356.1 | + | 30264 | 0.66 | 0.716192 |
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Target: 5'- -cGCAaugacccggccCGUCCGACaCAGACGGGg -3' miRNA: 3'- aaCGUguuu-------GCGGGCUGcGUUUGCCC- -5' |
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| 12728 | 5' | -53.4 | NC_003356.1 | + | 19161 | 0.67 | 0.644021 |
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Target: 5'- -gGCACGgcugcGGCGCUCGGCGgGgcGACGGu -3' miRNA: 3'- aaCGUGU-----UUGCGGGCUGCgU--UUGCCc -5' |
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| 12728 | 5' | -53.4 | NC_003356.1 | + | 29747 | 0.7 | 0.49862 |
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Target: 5'- cUGUACAcuggAGCGCCCGACauaCAGACucaGGGu -3' miRNA: 3'- aACGUGU----UUGCGGGCUGc--GUUUG---CCC- -5' |
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| 12728 | 5' | -53.4 | NC_003356.1 | + | 38476 | 0.71 | 0.426364 |
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Target: 5'- aUGCACAGACGCcugcaacacaCCGAUcCGGACGuGGg -3' miRNA: 3'- aACGUGUUUGCG----------GGCUGcGUUUGC-CC- -5' |
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| 12728 | 5' | -53.4 | NC_003356.1 | + | 29699 | 0.75 | 0.272511 |
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Target: 5'- gUGUACAggUGCCUGugGUGAAUGGc -3' miRNA: 3'- aACGUGUuuGCGGGCugCGUUUGCCc -5' |
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| 12728 | 5' | -53.4 | NC_003356.1 | + | 28210 | 1.08 | 0.001361 |
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Target: 5'- uUUGCACAAACGCCCGACGCAAACGGGc -3' miRNA: 3'- -AACGUGUUUGCGGGCUGCGUUUGCCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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