miRNA display CGI


Results 1 - 4 of 4 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
12729 5' -58.6 NC_003356.1 + 28584 1.06 0.000489
Target:  5'- cUGCGUCCAGUGACGCCGCCGCUCAAUa -3'
miRNA:   3'- -ACGCAGGUCACUGCGGCGGCGAGUUA- -5'
12729 5' -58.6 NC_003356.1 + 20762 0.68 0.335984
Target:  5'- cGCuaCCAGUGcccauuuCGCCGCCGUUCGc- -3'
miRNA:   3'- aCGcaGGUCACu------GCGGCGGCGAGUua -5'
12729 5' -58.6 NC_003356.1 + 38433 0.7 0.223244
Target:  5'- gGCGUgugacguugCCGGUgGAUGCCGgUGCUCAGUg -3'
miRNA:   3'- aCGCA---------GGUCA-CUGCGGCgGCGAGUUA- -5'
12729 5' -58.6 NC_003356.1 + 34363 0.66 0.415639
Target:  5'- cGCGUgCGGUGugGCCcacagauuCCGCaUCGGUu -3'
miRNA:   3'- aCGCAgGUCACugCGGc-------GGCG-AGUUA- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.