Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 12730 | 5' | -55.4 | NC_003356.1 | + | 25607 | 0.69 | 0.440356 |
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Target: 5'- aAGGCGCugccuGCGUUGCUCugGCAGCa- -3' miRNA: 3'- -UUUGCGcc---CGUAGCGAGugCGUUGcg -5' |
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| 12730 | 5' | -55.4 | NC_003356.1 | + | 9558 | 0.68 | 0.506621 |
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Target: 5'- cGACGCGccaGCGUCuuuuuuaucguccauGCUCGCGCA-CGCc -3' miRNA: 3'- uUUGCGCc--CGUAG---------------CGAGUGCGUuGCG- -5' |
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| 12730 | 5' | -55.4 | NC_003356.1 | + | 22758 | 0.67 | 0.533657 |
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Target: 5'- cAGGCGUGGGaaguuuuUCGUccagccagacugaUgGCGCAACGCa -3' miRNA: 3'- -UUUGCGCCCgu-----AGCG-------------AgUGCGUUGCG- -5' |
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| 12730 | 5' | -55.4 | NC_003356.1 | + | 13317 | 0.66 | 0.611598 |
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Target: 5'- -uACGCaggccagGGGCAUCGCagaaaucaauaUCACGaaaCAACGUa -3' miRNA: 3'- uuUGCG-------CCCGUAGCG-----------AGUGC---GUUGCG- -5' |
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| 12730 | 5' | -55.4 | NC_003356.1 | + | 28690 | 1.08 | 0.000698 |
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Target: 5'- aAAACGCGGGCAUCGCUCACGCAACGCc -3' miRNA: 3'- -UUUGCGCCCGUAGCGAGUGCGUUGCG- -5' |
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| 12730 | 5' | -55.4 | NC_003356.1 | + | 27396 | 0.72 | 0.276697 |
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Target: 5'- -uACGCGGGCAcaaaaaaaacCGCaUUACGCAGCGg -3' miRNA: 3'- uuUGCGCCCGUa---------GCG-AGUGCGUUGCg -5' |
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| 12730 | 5' | -55.4 | NC_003356.1 | + | 12487 | 0.7 | 0.364951 |
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Target: 5'- cAAUGC-GGCAUUGCgC-CGCAACGCu -3' miRNA: 3'- uUUGCGcCCGUAGCGaGuGCGUUGCG- -5' |
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| 12730 | 5' | -55.4 | NC_003356.1 | + | 25318 | 0.66 | 0.590218 |
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Target: 5'- ---aGCGGGauuucauGUCGC-CACGCAACa- -3' miRNA: 3'- uuugCGCCCg------UAGCGaGUGCGUUGcg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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