Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12733 | 3' | -55.6 | NC_003356.1 | + | 29545 | 0.66 | 0.660533 |
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Target: 5'- ---cUGCUGACGGCAUCAgcaCCaGGCu -3' miRNA: 3'- cgguACGGCUGCUGUAGUag-GGcCUG- -5' |
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| 12733 | 3' | -55.6 | NC_003356.1 | + | 2354 | 0.66 | 0.649414 |
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Target: 5'- aGUUAcUGUCGGCGGCaAUCugaCCGGACu -3' miRNA: 3'- -CGGU-ACGGCUGCUG-UAGuagGGCCUG- -5' |
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| 12733 | 3' | -55.6 | NC_003356.1 | + | 31695 | 0.66 | 0.649414 |
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Target: 5'- aGCgGUGCCGAC-ACcUUAUCCaCGG-Ca -3' miRNA: 3'- -CGgUACGGCUGcUGuAGUAGG-GCCuG- -5' |
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| 12733 | 3' | -55.6 | NC_003356.1 | + | 34840 | 0.66 | 0.615999 |
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Target: 5'- cGUCAUGguaCGACGAUG-CGUCCCGuGAa -3' miRNA: 3'- -CGGUACg--GCUGCUGUaGUAGGGC-CUg -5' |
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| 12733 | 3' | -55.6 | NC_003356.1 | + | 37903 | 0.67 | 0.571689 |
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Target: 5'- cGCCcuuCCGGC-ACAcCAUCCCGGAa -3' miRNA: 3'- -CGGuacGGCUGcUGUaGUAGGGCCUg -5' |
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| 12733 | 3' | -55.6 | NC_003356.1 | + | 35113 | 0.68 | 0.527109 |
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Target: 5'- uGCCggGCCaGCGggugguaauggcaACAUUaaaAUCCCGGGCa -3' miRNA: 3'- -CGGuaCGGcUGC-------------UGUAG---UAGGGCCUG- -5' |
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| 12733 | 3' | -55.6 | NC_003356.1 | + | 30981 | 1.12 | 0.000411 |
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Target: 5'- aGCCAUGCCGACGACAUCAUCCCGGACa -3' miRNA: 3'- -CGGUACGGCUGCUGUAGUAGGGCCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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