Results 1 - 20 of 22 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12734 | 5' | -52 | NC_003356.1 | + | 39493 | 0.66 | 0.831444 |
Target: 5'- aCCGCCcAGAcGCACAuccugUACACC--GCCu -3' miRNA: 3'- cGGCGGcUUU-CGUGU-----AUGUGGuaCGG- -5' |
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12734 | 5' | -52 | NC_003356.1 | + | 27117 | 0.66 | 0.831444 |
Target: 5'- uGCCGCCGAu-GCAaaa--GCCAUuGCg -3' miRNA: 3'- -CGGCGGCUuuCGUguaugUGGUA-CGg -5' |
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12734 | 5' | -52 | NC_003356.1 | + | 5781 | 0.66 | 0.82197 |
Target: 5'- aCCGCCGA----ACu--CGCCAUGCUg -3' miRNA: 3'- cGGCGGCUuucgUGuauGUGGUACGG- -5' |
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12734 | 5' | -52 | NC_003356.1 | + | 19614 | 0.66 | 0.816182 |
Target: 5'- aCCGCUGAugucauGGCugGccugguugcugaugcUGCugUAUGCCu -3' miRNA: 3'- cGGCGGCUu-----UCGugU---------------AUGugGUACGG- -5' |
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12734 | 5' | -52 | NC_003356.1 | + | 40190 | 0.66 | 0.81228 |
Target: 5'- -gCGCUGAuugauGGCGCAgGCAuccCCAUGUCc -3' miRNA: 3'- cgGCGGCUu----UCGUGUaUGU---GGUACGG- -5' |
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12734 | 5' | -52 | NC_003356.1 | + | 42285 | 0.66 | 0.792296 |
Target: 5'- aGCCGCgGuacGUucuuGCAUACGaCGUGCCg -3' miRNA: 3'- -CGGCGgCuuuCG----UGUAUGUgGUACGG- -5' |
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12734 | 5' | -52 | NC_003356.1 | + | 32457 | 0.67 | 0.760988 |
Target: 5'- uUCGCCaGAGaauccAGUACGUGCAgCAUGgCCc -3' miRNA: 3'- cGGCGG-CUU-----UCGUGUAUGUgGUAC-GG- -5' |
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12734 | 5' | -52 | NC_003356.1 | + | 40573 | 0.67 | 0.750249 |
Target: 5'- cGCUGCCGcuuccGCGCucugcUGCGCCugcGCCa -3' miRNA: 3'- -CGGCGGCuuu--CGUGu----AUGUGGua-CGG- -5' |
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12734 | 5' | -52 | NC_003356.1 | + | 37713 | 0.67 | 0.749167 |
Target: 5'- uGuuGCCGGAAGCGgauuguaUGUAUuCgGUGCCa -3' miRNA: 3'- -CggCGGCUUUCGU-------GUAUGuGgUACGG- -5' |
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12734 | 5' | -52 | NC_003356.1 | + | 24249 | 0.68 | 0.72839 |
Target: 5'- aCUGCCGu--GUACGgucgagAUAUCGUGCCa -3' miRNA: 3'- cGGCGGCuuuCGUGUa-----UGUGGUACGG- -5' |
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12734 | 5' | -52 | NC_003356.1 | + | 30444 | 0.68 | 0.72839 |
Target: 5'- uGCCGCCuuccacauAAGC-CGU-CGCCAcGCCg -3' miRNA: 3'- -CGGCGGcu------UUCGuGUAuGUGGUaCGG- -5' |
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12734 | 5' | -52 | NC_003356.1 | + | 41625 | 0.68 | 0.706114 |
Target: 5'- gGCCGCCaGAuaAAGCgACAUAUguACCAgGCa -3' miRNA: 3'- -CGGCGG-CU--UUCG-UGUAUG--UGGUaCGg -5' |
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12734 | 5' | -52 | NC_003356.1 | + | 22233 | 0.68 | 0.694852 |
Target: 5'- uGCCGCCGuGAGUGCcggguuuuucuUugGCCccGCCu -3' miRNA: 3'- -CGGCGGCuUUCGUGu----------AugUGGuaCGG- -5' |
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12734 | 5' | -52 | NC_003356.1 | + | 29546 | 0.69 | 0.672141 |
Target: 5'- uCUGCUGAcGGCAUcaGCACCAgGCUg -3' miRNA: 3'- cGGCGGCUuUCGUGuaUGUGGUaCGG- -5' |
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12734 | 5' | -52 | NC_003356.1 | + | 2504 | 0.69 | 0.672141 |
Target: 5'- gGCCGCCGGgcuaagggaaAAGCAgAUGaACCcccauaAUGCCc -3' miRNA: 3'- -CGGCGGCU----------UUCGUgUAUgUGG------UACGG- -5' |
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12734 | 5' | -52 | NC_003356.1 | + | 9822 | 0.72 | 0.481194 |
Target: 5'- uGCCuGCCacuuuAAGCGCAUGCACCAgaacaucGUCg -3' miRNA: 3'- -CGG-CGGcu---UUCGUGUAUGUGGUa------CGG- -5' |
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12734 | 5' | -52 | NC_003356.1 | + | 2705 | 0.73 | 0.416757 |
Target: 5'- aGCUGCCGGAcaGGCugAUGCGauucgcacaaaagcCCAgGCCg -3' miRNA: 3'- -CGGCGGCUU--UCGugUAUGU--------------GGUaCGG- -5' |
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12734 | 5' | -52 | NC_003356.1 | + | 23926 | 0.73 | 0.407019 |
Target: 5'- cGUCGCCGGgaaugugcuuuccaAucacguuGCugAUACACCAUGUCa -3' miRNA: 3'- -CGGCGGCU--------------Uu------CGugUAUGUGGUACGG- -5' |
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12734 | 5' | -52 | NC_003356.1 | + | 19333 | 0.74 | 0.390797 |
Target: 5'- cGCCagGCUGAAAGCGCcUGCGgCAuuguUGCCa -3' miRNA: 3'- -CGG--CGGCUUUCGUGuAUGUgGU----ACGG- -5' |
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12734 | 5' | -52 | NC_003356.1 | + | 19226 | 0.76 | 0.296482 |
Target: 5'- cCCGCCGA--GCGCcgcaGCCGUGCCg -3' miRNA: 3'- cGGCGGCUuuCGUGuaugUGGUACGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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