miRNA display CGI


Results 1 - 6 of 6 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
12735 3' -59.1 NC_003356.1 + 8746 0.68 0.335414
Target:  5'- cGCUGGgGCUGacaaauacugcUAUCuCCCUUCAGUc- -3'
miRNA:   3'- uCGACCgCGAC-----------GUAG-GGGAAGUCGcc -5'
12735 3' -59.1 NC_003356.1 + 21527 0.74 0.122631
Target:  5'- cGCUGGUGCUGCAcCCagCCUUCAccGCGa -3'
miRNA:   3'- uCGACCGCGACGUaGG--GGAAGU--CGCc -5'
12735 3' -59.1 NC_003356.1 + 21899 0.66 0.42384
Target:  5'- gAGCUGGCag-GCAaUCCCggcaAGCGGg -3'
miRNA:   3'- -UCGACCGcgaCGUaGGGGaag-UCGCC- -5'
12735 3' -59.1 NC_003356.1 + 31357 0.68 0.327348
Target:  5'- uGCUGGCGg-GgAUCCCgCguucaUCGGCGGc -3'
miRNA:   3'- uCGACCGCgaCgUAGGG-Ga----AGUCGCC- -5'
12735 3' -59.1 NC_003356.1 + 31717 1.09 0.000249
Target:  5'- cAGCUGGCGCUGCAUCCCCUUCAGCGGu -3'
miRNA:   3'- -UCGACCGCGACGUAGGGGAAGUCGCC- -5'
12735 3' -59.1 NC_003356.1 + 40768 0.68 0.319427
Target:  5'- cGGCcuguuUGGCgGCUucaGCAUCCUgccugacuuCUUCAGCGGu -3'
miRNA:   3'- -UCG-----ACCG-CGA---CGUAGGG---------GAAGUCGCC- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.