miRNA: 3'- -UCAAGGGGCGAGAGUUGUUUGACUUU- -5'
miRNA: 3'- -UCAAGGGgCGAGA-GUUGUUuGACuuu -5'
miRNA: 3'- -UCAAGGggCGAGa----GUUGUUUGACUUu -5'
Results 1 - 3 of 3 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 12737 | 3' | -49.4 | NC_003356.1 | + | 34019 | 1.07 | 0.003481 |
|
Target: 5'- cAGUUCCCCGCUCUCAACAAACUGAAAu -3' miRNA: 3'- -UCAAGGGGCGAGAGUUGUUUGACUUU- -5' |
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| 12737 | 3' | -49.4 | NC_003356.1 | + | 20150 | 0.67 | 0.852416 |
|
Target: 5'- cAGUUCCCaGCUCUgCGGCAGcCUGc-- -3' miRNA: 3'- -UCAAGGGgCGAGA-GUUGUUuGACuuu -5' |
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| 12737 | 3' | -49.4 | NC_003356.1 | + | 9744 | 0.71 | 0.644729 |
|
Target: 5'- aGGUUCCaaGCUCgcuggUGGCGGACUGAAc -3' miRNA: 3'- -UCAAGGggCGAGa----GUUGUUUGACUUu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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