miRNA display CGI


Results 1 - 7 of 7 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
12738 5' -52.5 NC_003356.1 + 42054 0.66 0.765282
Target:  5'- cGAUGCCGGAacuggaagaacUGGCGgCAGCaauGGcgCAg -3'
miRNA:   3'- aCUACGGUUU-----------ACCGC-GUCGg--UCaaGU- -5'
12738 5' -52.5 NC_003356.1 + 20008 0.66 0.754424
Target:  5'- gGAUGUC--GUGGCcuacauugGUAGCCAGgugUCAu -3'
miRNA:   3'- aCUACGGuuUACCG--------CGUCGGUCa--AGU- -5'
12738 5' -52.5 NC_003356.1 + 24139 0.66 0.754424
Target:  5'- uUGuUGCCGg--GGCGCuguGCCuGUUCu -3'
miRNA:   3'- -ACuACGGUuuaCCGCGu--CGGuCAAGu -5'
12738 5' -52.5 NC_003356.1 + 7736 0.66 0.743425
Target:  5'- cUGAUGCCuguGAUGGagaacaUAGCCAGUaCGg -3'
miRNA:   3'- -ACUACGGu--UUACCgc----GUCGGUCAaGU- -5'
12738 5' -52.5 NC_003356.1 + 27836 0.66 0.719933
Target:  5'- uUGAUGUCugggcaaAGGUGGCuCAGCCAGg--- -3'
miRNA:   3'- -ACUACGG-------UUUACCGcGUCGGUCaagu -5'
12738 5' -52.5 NC_003356.1 + 28635 0.73 0.33492
Target:  5'- cGAUGCCcgcguuuuGAggaucguguugGUGGCGCGGCgCAGUUUAa -3'
miRNA:   3'- aCUACGG--------UU-----------UACCGCGUCG-GUCAAGU- -5'
12738 5' -52.5 NC_003356.1 + 35357 1.09 0.001086
Target:  5'- gUGAUGCCAAAUGGCGCAGCCAGUUCAc -3'
miRNA:   3'- -ACUACGGUUUACCGCGUCGGUCAAGU- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.