Results 1 - 18 of 18 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
1274 | 5' | -54.3 | NC_001317.1 | + | 7034 | 0.66 | 0.604281 |
Target: 5'- uCGGCCAGuugcuuaaacccGGC-GGC-AGUCUGGCGCa -3' miRNA: 3'- uGUUGGUC------------UUGcCCGuUCGGACUGCG- -5' |
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1274 | 5' | -54.3 | NC_001317.1 | + | 19935 | 0.66 | 0.604281 |
Target: 5'- gACGGCCAGc---GGCAAGCCcGuccCGCa -3' miRNA: 3'- -UGUUGGUCuugcCCGUUCGGaCu--GCG- -5' |
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1274 | 5' | -54.3 | NC_001317.1 | + | 10656 | 0.66 | 0.592711 |
Target: 5'- aGCAucACCAGucucaaagcuGAUGGauGUAAGCCUGAuaCGCg -3' miRNA: 3'- -UGU--UGGUC----------UUGCC--CGUUCGGACU--GCG- -5' |
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1274 | 5' | -54.3 | NC_001317.1 | + | 9374 | 0.66 | 0.581178 |
Target: 5'- cCAGCCGGuACGGaGCAagaaaGGCCgcauuaaGCGCg -3' miRNA: 3'- uGUUGGUCuUGCC-CGU-----UCGGac-----UGCG- -5' |
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1274 | 5' | -54.3 | NC_001317.1 | + | 9929 | 0.66 | 0.569692 |
Target: 5'- gGCAAUCGGu---GGCGAGCUUGAuacCGCu -3' miRNA: 3'- -UGUUGGUCuugcCCGUUCGGACU---GCG- -5' |
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1274 | 5' | -54.3 | NC_001317.1 | + | 9911 | 0.66 | 0.569692 |
Target: 5'- aACGACCGGcGCGGGUGgucagccaguucAGCCaUGuCGUc -3' miRNA: 3'- -UGUUGGUCuUGCCCGU------------UCGG-ACuGCG- -5' |
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1274 | 5' | -54.3 | NC_001317.1 | + | 20445 | 0.66 | 0.558263 |
Target: 5'- aGCGGCUucacGACGGGCuuaGAGCUUGAgGUu -3' miRNA: 3'- -UGUUGGuc--UUGCCCG---UUCGGACUgCG- -5' |
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1274 | 5' | -54.3 | NC_001317.1 | + | 9549 | 0.67 | 0.513289 |
Target: 5'- aACAGCCGGGAUGuGCAGuaCgaGGCGCg -3' miRNA: 3'- -UGUUGGUCUUGCcCGUUcgGa-CUGCG- -5' |
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1274 | 5' | -54.3 | NC_001317.1 | + | 25274 | 0.67 | 0.513289 |
Target: 5'- uAUAugCAGAGuauCGcGGCGAGCCagGGCGa -3' miRNA: 3'- -UGUugGUCUU---GC-CCGUUCGGa-CUGCg -5' |
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1274 | 5' | -54.3 | NC_001317.1 | + | 20314 | 0.68 | 0.480555 |
Target: 5'- uUAGCCuGAGCGGcacGCAucGCCUG-CGCc -3' miRNA: 3'- uGUUGGuCUUGCC---CGUu-CGGACuGCG- -5' |
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1274 | 5' | -54.3 | NC_001317.1 | + | 17689 | 0.68 | 0.46987 |
Target: 5'- aACAACCu---CGGGaCAAGCCa-GCGCg -3' miRNA: 3'- -UGUUGGucuuGCCC-GUUCGGacUGCG- -5' |
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1274 | 5' | -54.3 | NC_001317.1 | + | 12484 | 0.68 | 0.468809 |
Target: 5'- -aAGCCAGAACuugccgaGGGCucAGGCCguGCGCa -3' miRNA: 3'- ugUUGGUCUUG-------CCCG--UUCGGacUGCG- -5' |
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1274 | 5' | -54.3 | NC_001317.1 | + | 27728 | 0.7 | 0.343575 |
Target: 5'- aACAGCaaauGGAACGGuGCAAGCCcGucagacACGCa -3' miRNA: 3'- -UGUUGg---UCUUGCC-CGUUCGGaC------UGCG- -5' |
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1274 | 5' | -54.3 | NC_001317.1 | + | 2145 | 0.7 | 0.338368 |
Target: 5'- cAUGACCAGcAGCGGGCGcagcgccaccuacgaGGCCaGcCGCa -3' miRNA: 3'- -UGUUGGUC-UUGCCCGU---------------UCGGaCuGCG- -5' |
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1274 | 5' | -54.3 | NC_001317.1 | + | 9278 | 0.7 | 0.334929 |
Target: 5'- gGCAAUCAGAccgGCGaGGCGcuCCUGcaGCGCg -3' miRNA: 3'- -UGUUGGUCU---UGC-CCGUucGGAC--UGCG- -5' |
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1274 | 5' | -54.3 | NC_001317.1 | + | 2417 | 0.7 | 0.334929 |
Target: 5'- uGCcACCAGAaaccaccgGCGGcGCGAGCaC-GACGCa -3' miRNA: 3'- -UGuUGGUCU--------UGCC-CGUUCG-GaCUGCG- -5' |
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1274 | 5' | -54.3 | NC_001317.1 | + | 6505 | 0.75 | 0.164262 |
Target: 5'- aGCGACUGGAACGGGCAuugaAGCCaaaGACa- -3' miRNA: 3'- -UGUUGGUCUUGCCCGU----UCGGa--CUGcg -5' |
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1274 | 5' | -54.3 | NC_001317.1 | + | 30555 | 1.12 | 0.000314 |
Target: 5'- cACAACCAGAACGGGCAAGCCUGACGCa -3' miRNA: 3'- -UGUUGGUCUUGCCCGUUCGGACUGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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