miRNA display CGI


Results 1 - 7 of 7 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
12744 5' -48.9 NC_003356.1 + 30790 0.77 0.397103
Target:  5'- cGGCgggaACAGCGuguuCAGuCGGUUCGGGUUAu -3'
miRNA:   3'- -CCG----UGUUGUu---GUCuGUCAAGCCCAAU- -5'
12744 5' -48.9 NC_003356.1 + 21417 1.1 0.002697
Target:  5'- aGGCACAACAACAGACAGUUCGGGUUAc -3'
miRNA:   3'- -CCGUGUUGUUGUCUGUCAAGCCCAAU- -5'
12744 5' -48.9 NC_003356.1 + 35901 0.71 0.688359
Target:  5'- gGGCACGACGACuGAauua-CGGGUUGg -3'
miRNA:   3'- -CCGUGUUGUUGuCUgucaaGCCCAAU- -5'
12744 5' -48.9 NC_003356.1 + 39328 0.68 0.866314
Target:  5'- uGGCGCGuuACAAaguaacCGGGCAGUgaauguuucaggUCGGGUa- -3'
miRNA:   3'- -CCGUGU--UGUU------GUCUGUCA------------AGCCCAau -5'
12744 5' -48.9 NC_003356.1 + 26578 0.67 0.883014
Target:  5'- cGGCACAACGucCGGAUGGgUgGGGg-- -3'
miRNA:   3'- -CCGUGUUGUu-GUCUGUCaAgCCCaau -5'
12744 5' -48.9 NC_003356.1 + 19160 0.67 0.89628
Target:  5'- cGGCACGGCuGCGgcgcucggcggggcGACGGUauUCGGGc-- -3'
miRNA:   3'- -CCGUGUUGuUGU--------------CUGUCA--AGCCCaau -5'
12744 5' -48.9 NC_003356.1 + 29616 0.66 0.931873
Target:  5'- aGGgGCGGCGGC-GACGGUcaGGGUg- -3'
miRNA:   3'- -CCgUGUUGUUGuCUGUCAagCCCAau -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.