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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12745 | 5' | -55.7 | NC_003356.1 | + | 21668 | 1.08 | 0.000615 |
Target: 5'- aGAGUCUGUGCUGGCCCUGCCACAAAGc -3' miRNA: 3'- -CUCAGACACGACCGGGACGGUGUUUC- -5' |
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12745 | 5' | -55.7 | NC_003356.1 | + | 18998 | 0.71 | 0.286394 |
Target: 5'- uGGUgCUGUGCUGGCCgUGCUcuggccucuacugcGCAAAa -3' miRNA: 3'- cUCA-GACACGACCGGgACGG--------------UGUUUc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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