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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 12748 | 5' | -58.6 | NC_003356.1 | + | 21467 | 0.68 | 0.320088 |
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Target: 5'- gCUGgguGCAGCACCaGCGcGGAcUGAGCCg- -3' miRNA: 3'- -GAC---CGUCGUGG-CGUaCCU-ACUCGGgu -5' |
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| 12748 | 5' | -58.6 | NC_003356.1 | + | 25626 | 1.08 | 0.000332 |
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Target: 5'- uCUGGCAGCACCGCAUGGAUGAGCCCAu -3' miRNA: 3'- -GACCGUCGUGGCGUACCUACUCGGGU- -5' |
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| 12748 | 5' | -58.6 | NC_003356.1 | + | 27915 | 0.67 | 0.361652 |
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Target: 5'- -cGGCAGCGCC---UGGcUGAGCCa- -3' miRNA: 3'- gaCCGUCGUGGcguACCuACUCGGgu -5' |
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| 12748 | 5' | -58.6 | NC_003356.1 | + | 29510 | 0.67 | 0.370402 |
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Target: 5'- aUGGCGGCAUCGUugcUGGcau-GCCCGa -3' miRNA: 3'- gACCGUCGUGGCGu--ACCuacuCGGGU- -5' |
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| 12748 | 5' | -58.6 | NC_003356.1 | + | 31639 | 0.68 | 0.29692 |
|
Target: 5'- -aGGUgucGGCACCGCugaaggGGAUGcAGCgCCAg -3' miRNA: 3'- gaCCG---UCGUGGCGua----CCUAC-UCG-GGU- -5' |
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| 12748 | 5' | -58.6 | NC_003356.1 | + | 34352 | 0.75 | 0.111662 |
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Target: 5'- -aGGacuGGCACCGCGUGcGGUGuGGCCCAc -3' miRNA: 3'- gaCCg--UCGUGGCGUAC-CUAC-UCGGGU- -5' |
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| 12748 | 5' | -58.6 | NC_003356.1 | + | 35326 | 0.67 | 0.388333 |
|
Target: 5'- gCUGGC-GUGCCGCAgacagcGGugauUGAuGCCCAg -3' miRNA: 3'- -GACCGuCGUGGCGUa-----CCu---ACU-CGGGU- -5' |
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| 12748 | 5' | -58.6 | NC_003356.1 | + | 40791 | 0.66 | 0.406824 |
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Target: 5'- uCUGGCcuGCuCUGCAUcGGAUGcGGCCUg -3' miRNA: 3'- -GACCGu-CGuGGCGUA-CCUAC-UCGGGu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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