Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 12749 | 3' | -51.8 | NC_003356.1 | + | 26782 | 1.04 | 0.00246 |
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Target: 5'- cUUUACCAGUGGCGACGGCAGCAAAAAa -3' miRNA: 3'- -AAAUGGUCACCGCUGCCGUCGUUUUU- -5' |
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| 12749 | 3' | -51.8 | NC_003356.1 | + | 29610 | 0.7 | 0.523858 |
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Target: 5'- --aGCCAGaGG-GGCGGCGGCGAc-- -3' miRNA: 3'- aaaUGGUCaCCgCUGCCGUCGUUuuu -5' |
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| 12749 | 3' | -51.8 | NC_003356.1 | + | 42056 | 0.67 | 0.676478 |
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Target: 5'- -aUGCCGGaacUGGaagaacuGGCGGCAGCAAu-- -3' miRNA: 3'- aaAUGGUC---ACCg------CUGCCGUCGUUuuu -5' |
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| 12749 | 3' | -51.8 | NC_003356.1 | + | 22244 | 0.67 | 0.688226 |
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Target: 5'- -cUGCCAGUcGGCaGCGGCuuaugacgccgaAGCAGGGGa -3' miRNA: 3'- aaAUGGUCA-CCGcUGCCG------------UCGUUUUU- -5' |
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| 12749 | 3' | -51.8 | NC_003356.1 | + | 30361 | 0.67 | 0.69991 |
|
Target: 5'- uUUUAUCucacggcGUGGCGACGGCuuauGUGGAAGg -3' miRNA: 3'- -AAAUGGu------CACCGCUGCCGu---CGUUUUU- -5' |
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| 12749 | 3' | -51.8 | NC_003356.1 | + | 41403 | 0.66 | 0.7569 |
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Target: 5'- -aUACCuaccGGaCGAUGGCAGCAGu-- -3' miRNA: 3'- aaAUGGuca-CC-GCUGCCGUCGUUuuu -5' |
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| 12749 | 3' | -51.8 | NC_003356.1 | + | 39648 | 0.66 | 0.767911 |
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Target: 5'- -aUACCAGcUGGCG-CGaCAGCAuAAAu -3' miRNA: 3'- aaAUGGUC-ACCGCuGCcGUCGUuUUU- -5' |
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| 12749 | 3' | -51.8 | NC_003356.1 | + | 11713 | 0.69 | 0.593613 |
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Target: 5'- --gGCCAGUucuccgcauagcGGCGACaGGCcGCAAGAu -3' miRNA: 3'- aaaUGGUCA------------CCGCUG-CCGuCGUUUUu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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