miRNA display CGI


Results 1 - 7 of 7 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
12750 5' -48.3 NC_003356.1 + 5174 0.67 0.94543
Target:  5'- --cACGCGGCUacGUGugGUCGUgcGCCg -3'
miRNA:   3'- guuUGCGCCGA--UAUggUAGCAacUGG- -5'
12750 5' -48.3 NC_003356.1 + 15757 0.66 0.950334
Target:  5'- cCGAAC-CGGCg--ACCGUCGggGAa- -3'
miRNA:   3'- -GUUUGcGCCGauaUGGUAGCaaCUgg -5'
12750 5' -48.3 NC_003356.1 + 20034 0.67 0.922713
Target:  5'- aGGugGCGGCUGU-UCAUgGUcUGGCUu -3'
miRNA:   3'- gUUugCGCCGAUAuGGUAgCA-ACUGG- -5'
12750 5' -48.3 NC_003356.1 + 26443 0.71 0.7745
Target:  5'- aGAugGCGGUUAUGCCAUucCGgaagaacugGAUCg -3'
miRNA:   3'- gUUugCGCCGAUAUGGUA--GCaa-------CUGG- -5'
12750 5' -48.3 NC_003356.1 + 27152 1.14 0.002032
Target:  5'- uCAAACGCGGCUAUACCAUCGUUGACCg -3'
miRNA:   3'- -GUUUGCGCCGAUAUGGUAGCAACUGG- -5'
12750 5' -48.3 NC_003356.1 + 27188 0.7 0.825649
Target:  5'- cCAAACGCaauGGCUuUugCAUCGgcGGCa -3'
miRNA:   3'- -GUUUGCG---CCGAuAugGUAGCaaCUGg -5'
12750 5' -48.3 NC_003356.1 + 32084 0.68 0.909478
Target:  5'- --uGCGCcaCUGUGCCAUCaucUGACCg -3'
miRNA:   3'- guuUGCGccGAUAUGGUAGca-ACUGG- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.