miRNA display CGI


Results 1 - 6 of 6 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
1276 3' -55.9 NC_001317.1 + 1199 1.09 0.000273
Target:  5'- gUGGCUUGCGCUUGCUCAUGCUGCCACc -3'
miRNA:   3'- -ACCGAACGCGAACGAGUACGACGGUG- -5'
1276 3' -55.9 NC_001317.1 + 2500 0.69 0.260654
Target:  5'- cUGGCgacaGCGCcgggUGCgUCGUGCUcgcGCCGCc -3'
miRNA:   3'- -ACCGaa--CGCGa---ACG-AGUACGA---CGGUG- -5'
1276 3' -55.9 NC_001317.1 + 27874 0.67 0.395809
Target:  5'- cUGGCauaUGCUuaUGUUCAUGUUGCCGg -3'
miRNA:   3'- -ACCGaacGCGA--ACGAGUACGACGGUg -5'
1276 3' -55.9 NC_001317.1 + 24747 0.66 0.435975
Target:  5'- cGGCUgcGCGCgUUGCUUAUGUgGCguuCGCg -3'
miRNA:   3'- aCCGAa-CGCG-AACGAGUACGaCG---GUG- -5'
1276 3' -55.9 NC_001317.1 + 9937 0.66 0.446379
Target:  5'- gUGGCga--GCUUGauaccgcuUUCGUGCUGCCGg -3'
miRNA:   3'- -ACCGaacgCGAAC--------GAGUACGACGGUg -5'
1276 3' -55.9 NC_001317.1 + 27364 0.66 0.45692
Target:  5'- gUGGCgcgGcCGCUUGCgacgCAuUGCgGCUGCa -3'
miRNA:   3'- -ACCGaa-C-GCGAACGa---GU-ACGaCGGUG- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.