Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1277 | 3' | -54.1 | NC_001317.1 | + | 27924 | 0.66 | 0.575259 |
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Target: 5'- cAUGaACAUaaGCauaUGCCAGUGCGGCGu -3' miRNA: 3'- cUAUcUGUAa-UGg--GCGGUCACGCCGC- -5' |
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| 1277 | 3' | -54.1 | NC_001317.1 | + | 11205 | 0.67 | 0.51855 |
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Target: 5'- ---cGACAc-GCCCGUCAGcacUGCGGCc -3' miRNA: 3'- cuauCUGUaaUGGGCGGUC---ACGCCGc -5' |
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| 1277 | 3' | -54.1 | NC_001317.1 | + | 6647 | 0.68 | 0.463131 |
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Target: 5'- uGGUGGACGcccgcgcUUugCUGCCGGUuugGUGGCa -3' miRNA: 3'- -CUAUCUGU-------AAugGGCGGUCA---CGCCGc -5' |
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| 1277 | 3' | -54.1 | NC_001317.1 | + | 17491 | 0.69 | 0.422922 |
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Target: 5'- ---cGGCAUuuuUugCCaCCGGUGCGGCa -3' miRNA: 3'- cuauCUGUA---AugGGcGGUCACGCCGc -5' |
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| 1277 | 3' | -54.1 | NC_001317.1 | + | 15 | 0.69 | 0.412953 |
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Target: 5'- ----aGCAUUGCgCGCCAGagGUGGCGc -3' miRNA: 3'- cuaucUGUAAUGgGCGGUCa-CGCCGC- -5' |
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| 1277 | 3' | -54.1 | NC_001317.1 | + | 7615 | 0.71 | 0.305344 |
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Target: 5'- ---uGACAUgucacUGCCCGCCGuGUGCGGg- -3' miRNA: 3'- cuauCUGUA-----AUGGGCGGU-CACGCCgc -5' |
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| 1277 | 3' | -54.1 | NC_001317.1 | + | 18740 | 0.73 | 0.220585 |
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Target: 5'- --aAGAgCGU--CCCGCCGGUGCGGUa -3' miRNA: 3'- cuaUCU-GUAauGGGCGGUCACGCCGc -5' |
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| 1277 | 3' | -54.1 | NC_001317.1 | + | 1575 | 1.08 | 0.000649 |
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Target: 5'- aGAUAGACAUUACCCGCCAGUGCGGCGc -3' miRNA: 3'- -CUAUCUGUAAUGGGCGGUCACGCCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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