Results 1 - 13 of 13 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12778 | 3' | -58.3 | NC_003387.1 | + | 11386 | 0.66 | 0.537611 |
Target: 5'- uUGCGGcgaucGGCGCCacgcuggccgcgaucGGCCCCgaggugcugUCGUCGGUg -3' miRNA: 3'- cACGCC-----UUGUGG---------------CCGGGG---------AGUAGUCA- -5' |
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12778 | 3' | -58.3 | NC_003387.1 | + | 31349 | 0.66 | 0.532335 |
Target: 5'- -cGCGGGucgaacaGCACCGGCCagggcagCGUCGGc -3' miRNA: 3'- caCGCCU-------UGUGGCCGGgga----GUAGUCa -5' |
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12778 | 3' | -58.3 | NC_003387.1 | + | 8056 | 0.66 | 0.512463 |
Target: 5'- -gGC-GAGCACCGgcaGCCCCUCGgcCAGg -3' miRNA: 3'- caCGcCUUGUGGC---CGGGGAGUa-GUCa -5' |
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12778 | 3' | -58.3 | NC_003387.1 | + | 27340 | 0.66 | 0.502127 |
Target: 5'- --uCGGAGCACCaGGCCCUcaaCAUCAa- -3' miRNA: 3'- cacGCCUUGUGG-CCGGGGa--GUAGUca -5' |
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12778 | 3' | -58.3 | NC_003387.1 | + | 24857 | 0.67 | 0.471694 |
Target: 5'- gGUGCGuGGugGCUGGCUcgaCCUCGggCGGUg -3' miRNA: 3'- -CACGC-CUugUGGCCGG---GGAGUa-GUCA- -5' |
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12778 | 3' | -58.3 | NC_003387.1 | + | 38688 | 0.67 | 0.442222 |
Target: 5'- aGUGCGGGuGCGCCGGauuCUCCUCGg-GGUa -3' miRNA: 3'- -CACGCCU-UGUGGCC---GGGGAGUagUCA- -5' |
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12778 | 3' | -58.3 | NC_003387.1 | + | 26846 | 0.68 | 0.413821 |
Target: 5'- -cGgGGAGCACCaGGCCgCUCGgcaacugCAGg -3' miRNA: 3'- caCgCCUUGUGG-CCGGgGAGUa------GUCa -5' |
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12778 | 3' | -58.3 | NC_003387.1 | + | 47400 | 0.69 | 0.369072 |
Target: 5'- -cGCGGGGCaACCGGCCCCg------- -3' miRNA: 3'- caCGCCUUG-UGGCCGGGGaguaguca -5' |
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12778 | 3' | -58.3 | NC_003387.1 | + | 8940 | 0.69 | 0.360529 |
Target: 5'- -aGCGGGcgACGCCGGUCCUggugUCGUCcuGGUa -3' miRNA: 3'- caCGCCU--UGUGGCCGGGG----AGUAG--UCA- -5' |
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12778 | 3' | -58.3 | NC_003387.1 | + | 37531 | 0.7 | 0.297236 |
Target: 5'- -aGCGGccgGGCGCgGGCCuCCUCGUCGa- -3' miRNA: 3'- caCGCC---UUGUGgCCGG-GGAGUAGUca -5' |
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12778 | 3' | -58.3 | NC_003387.1 | + | 16564 | 0.71 | 0.26897 |
Target: 5'- -gGCGGugAGCAuCCGGCCgaccgCCUCGUCGGg -3' miRNA: 3'- caCGCC--UUGU-GGCCGG-----GGAGUAGUCa -5' |
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12778 | 3' | -58.3 | NC_003387.1 | + | 18915 | 0.72 | 0.224775 |
Target: 5'- -aGCGGGAUGCCcuGCgCCUCAUCGGg -3' miRNA: 3'- caCGCCUUGUGGc-CGgGGAGUAGUCa -5' |
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12778 | 3' | -58.3 | NC_003387.1 | + | 50198 | 1.07 | 0.000659 |
Target: 5'- cGUGCGGAACACCGGCCCCUCAUCAGUc -3' miRNA: 3'- -CACGCCUUGUGGCCGGGGAGUAGUCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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