miRNA: 3'- -AGUGGCCGa----CGACGCa--CCGGaCGGGC- -5'
miRNA: 3'- aGugGCCG---ACGACGCACCGG--AC-GGGC- -5'
miRNA: 3'- aGUGGCCGaCgACGCACCGG----ACGGGc -5'
miRNA: 3'- -AGUGG-CCG-ACGa-CGCA-------------CCGGACGGGC- -5'
miRNA: 3'- -AGUGGCCGACGACGCACCGGACGGGC- -5'
Results 41 - 45 of 45 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12779 | 3' | -63.2 | NC_003387.1 | + | 45377 | 0.74 | 0.078722 |
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Target: 5'- uUUGCCGGCgguuaGCUGCGcccGGCC-GCCCGa -3' miRNA: 3'- -AGUGGCCGa----CGACGCa--CCGGaCGGGC- -5' |
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| 12779 | 3' | -63.2 | NC_003387.1 | + | 51681 | 0.74 | 0.076582 |
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Target: 5'- gUugCGGCccuUGCUGCGUGGCUggUGaCCCGc -3' miRNA: 3'- aGugGCCG---ACGACGCACCGG--AC-GGGC- -5' |
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| 12779 | 3' | -63.2 | NC_003387.1 | + | 46606 | 0.76 | 0.053378 |
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Target: 5'- gCACCGGCaGgUGCGUGGUCuuggUGCCCa -3' miRNA: 3'- aGUGGCCGaCgACGCACCGG----ACGGGc -5' |
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| 12779 | 3' | -63.2 | NC_003387.1 | + | 24109 | 0.77 | 0.046279 |
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Target: 5'- aUCGCCuGGCaUGCggGCGUcggcagcuaucccGGCCUGCCCGa -3' miRNA: 3'- -AGUGG-CCG-ACGa-CGCA-------------CCGGACGGGC- -5' |
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| 12779 | 3' | -63.2 | NC_003387.1 | + | 49989 | 1.07 | 0.000197 |
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Target: 5'- uUCACCGGCUGCUGCGUGGCCUGCCCGc -3' miRNA: 3'- -AGUGGCCGACGACGCACCGGACGGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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