Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12786 | 5' | -55.3 | NC_003387.1 | + | 39223 | 0.66 | 0.687516 |
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Target: 5'- --aCUCCaccggCgaaCCCGCCGGGGcugUCGGu -3' miRNA: 3'- aaaGAGGaaa--Ga--GGGCGGCUCC---AGCC- -5' |
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| 12786 | 5' | -55.3 | NC_003387.1 | + | 33950 | 0.67 | 0.610558 |
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Target: 5'- --gCUUCggcagCuuGCCGGGGUCGGg -3' miRNA: 3'- aaaGAGGaaagaGggCGGCUCCAGCC- -5' |
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| 12786 | 5' | -55.3 | NC_003387.1 | + | 44981 | 0.68 | 0.577659 |
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Target: 5'- -aUCgCCg--CUgCCGCCGAGG-CGGu -3' miRNA: 3'- aaAGaGGaaaGAgGGCGGCUCCaGCC- -5' |
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| 12786 | 5' | -55.3 | NC_003387.1 | + | 38379 | 0.7 | 0.442212 |
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Target: 5'- --gCUCCUcgauccgCUCCgCGuuGAGGUUGGg -3' miRNA: 3'- aaaGAGGAaa-----GAGG-GCggCUCCAGCC- -5' |
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| 12786 | 5' | -55.3 | NC_003387.1 | + | 27134 | 0.74 | 0.274248 |
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Target: 5'- -gUCgaggCCgug--CCCGCCGGGGUCGGc -3' miRNA: 3'- aaAGa---GGaaagaGGGCGGCUCCAGCC- -5' |
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| 12786 | 5' | -55.3 | NC_003387.1 | + | 46760 | 1.07 | 0.001251 |
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Target: 5'- cUUUCUCCUUUCUCCCGCCGAGGUCGGu -3' miRNA: 3'- -AAAGAGGAAAGAGGGCGGCUCCAGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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