Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12787 | 5' | -51 | NC_003387.1 | + | 24839 | 0.66 | 0.917229 |
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Target: 5'- gUGCGCUGUcUGCGGcc-----GGGUGCg -3' miRNA: 3'- -ACGCGGCA-AUGCCaacuucuUCCACG- -5' |
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| 12787 | 5' | -51 | NC_003387.1 | + | 34316 | 0.66 | 0.896786 |
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Target: 5'- cGCGCCuUUcCGGUUGAacAGGuAGGcGCc -3' miRNA: 3'- aCGCGGcAAuGCCAACU--UCU-UCCaCG- -5' |
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| 12787 | 5' | -51 | NC_003387.1 | + | 34849 | 0.67 | 0.854517 |
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Target: 5'- cGCGCgGgccuUGCGGU--GAGAgcagccggaaaugcAGGUGCg -3' miRNA: 3'- aCGCGgCa---AUGCCAacUUCU--------------UCCACG- -5' |
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| 12787 | 5' | -51 | NC_003387.1 | + | 22247 | 0.67 | 0.848386 |
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Target: 5'- gGCGCCG--ACGG--GAAGcacuuGGUGCa -3' miRNA: 3'- aCGCGGCaaUGCCaaCUUCuu---CCACG- -5' |
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| 12787 | 5' | -51 | NC_003387.1 | + | 39156 | 0.67 | 0.848386 |
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Target: 5'- cGCGCCGaggGCGuGUacuGGucGGUGCg -3' miRNA: 3'- aCGCGGCaa-UGC-CAacuUCuuCCACG- -5' |
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| 12787 | 5' | -51 | NC_003387.1 | + | 38892 | 0.69 | 0.79133 |
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Target: 5'- cGCGCCGaacCG--UGGAGcGGGUGCg -3' miRNA: 3'- aCGCGGCaauGCcaACUUCuUCCACG- -5' |
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| 12787 | 5' | -51 | NC_003387.1 | + | 33771 | 0.69 | 0.781133 |
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Target: 5'- gGCGCCGcgaGCGGUUuGGcccGGcuGGUGCc -3' miRNA: 3'- aCGCGGCaa-UGCCAA-CU---UCuuCCACG- -5' |
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| 12787 | 5' | -51 | NC_003387.1 | + | 47534 | 0.69 | 0.770771 |
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Target: 5'- gGCGaCCGUUACGGc-GAGGuc-GUGCu -3' miRNA: 3'- aCGC-GGCAAUGCCaaCUUCuucCACG- -5' |
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| 12787 | 5' | -51 | NC_003387.1 | + | 29965 | 0.77 | 0.338568 |
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Target: 5'- cGCGCCGUUucgcgagguauccGCaGGUgaGAGggGGUGCu -3' miRNA: 3'- aCGCGGCAA-------------UG-CCAacUUCuuCCACG- -5' |
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| 12787 | 5' | -51 | NC_003387.1 | + | 46084 | 1.14 | 0.001238 |
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Target: 5'- aUGCGCCGUUACGGUUGAAGAAGGUGCg -3' miRNA: 3'- -ACGCGGCAAUGCCAACUUCUUCCACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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