Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 12797 | 5' | -58.9 | NC_003387.1 | + | 21945 | 0.67 | 0.423382 |
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Target: 5'- -gCGGGGgcGGCGgcaggcgUCUGCGG-CGCUg -3' miRNA: 3'- uaGCCCCauUCGCa------GGACGCUgGCGG- -5' |
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| 12797 | 5' | -58.9 | NC_003387.1 | + | 7193 | 0.66 | 0.501451 |
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Target: 5'- --aGcGGGUGguGGCGUCaaugGUGGCCGCg -3' miRNA: 3'- uagC-CCCAU--UCGCAGga--CGCUGGCGg -5' |
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| 12797 | 5' | -58.9 | NC_003387.1 | + | 7524 | 0.66 | 0.501451 |
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Target: 5'- -cCGaGGGUgaacgaGAGCGgcaCUGCuGCCGCCc -3' miRNA: 3'- uaGC-CCCA------UUCGCag-GACGcUGGCGG- -5' |
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| 12797 | 5' | -58.9 | NC_003387.1 | + | 9525 | 0.66 | 0.532345 |
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Target: 5'- -cCGuGGccGUcAAGCGgugCCUGCGcaaGCCGCCg -3' miRNA: 3'- uaGC-CC--CA-UUCGCa--GGACGC---UGGCGG- -5' |
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| 12797 | 5' | -58.9 | NC_003387.1 | + | 29213 | 0.66 | 0.511667 |
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Target: 5'- uUCGGGGUcGGCGcgguuggCCggGCaccagaACCGCCg -3' miRNA: 3'- uAGCCCCAuUCGCa------GGa-CGc-----UGGCGG- -5' |
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| 12797 | 5' | -58.9 | NC_003387.1 | + | 42741 | 0.66 | 0.480302 |
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Target: 5'- gGUCGGGu---GCGcCCUGCGGCggauucuuaugcgCGCCc -3' miRNA: 3'- -UAGCCCcauuCGCaGGACGCUG-------------GCGG- -5' |
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| 12797 | 5' | -58.9 | NC_003387.1 | + | 30255 | 0.66 | 0.491328 |
|
Target: 5'- cGUCGGGGUcGGCG-CCgggGUcgGGCaGCCa -3' miRNA: 3'- -UAGCCCCAuUCGCaGGa--CG--CUGgCGG- -5' |
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| 12797 | 5' | -58.9 | NC_003387.1 | + | 16205 | 0.66 | 0.511667 |
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Target: 5'- uGUCGGGGcuca-G-CCUGCG-CUGCCa -3' miRNA: 3'- -UAGCCCCauucgCaGGACGCuGGCGG- -5' |
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| 12797 | 5' | -58.9 | NC_003387.1 | + | 32844 | 0.66 | 0.532345 |
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Target: 5'- -gCGGGGcgGGGCGguUCC-GCG-UCGCCg -3' miRNA: 3'- uaGCCCCa-UUCGC--AGGaCGCuGGCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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