Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12797 | 5' | -58.9 | NC_003387.1 | + | 3183 | 0.71 | 0.270566 |
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Target: 5'- aAUCGGGGUGAGCcugcUCgggCUGCGACguccaGCCc -3' miRNA: 3'- -UAGCCCCAUUCGc---AG---GACGCUGg----CGG- -5' |
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| 12797 | 5' | -58.9 | NC_003387.1 | + | 51400 | 0.71 | 0.237906 |
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Target: 5'- uUCGGccGGUgaccggcGAGCG-CCUGCGgcGCCGCCu -3' miRNA: 3'- uAGCC--CCA-------UUCGCaGGACGC--UGGCGG- -5' |
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| 12797 | 5' | -58.9 | NC_003387.1 | + | 31013 | 0.72 | 0.226609 |
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Target: 5'- gAUCGGGccGAGCGUgcgCCgggGCGGCgGCCa -3' miRNA: 3'- -UAGCCCcaUUCGCA---GGa--CGCUGgCGG- -5' |
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| 12797 | 5' | -58.9 | NC_003387.1 | + | 51372 | 0.72 | 0.209699 |
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Target: 5'- cGUCGGGGUcGGCGUCCU-CGuCgGCg -3' miRNA: 3'- -UAGCCCCAuUCGCAGGAcGCuGgCGg -5' |
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| 12797 | 5' | -58.9 | NC_003387.1 | + | 44874 | 0.74 | 0.173051 |
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Target: 5'- cGUCGGGGgcgucGGCGauuccgCCUGCGcgccgggcaccucgGCCGCCa -3' miRNA: 3'- -UAGCCCCau---UCGCa-----GGACGC--------------UGGCGG- -5' |
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| 12797 | 5' | -58.9 | NC_003387.1 | + | 43134 | 0.75 | 0.14069 |
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Target: 5'- -gCGGuGGUGGGCGuUCCUGC-ACCGCa -3' miRNA: 3'- uaGCC-CCAUUCGC-AGGACGcUGGCGg -5' |
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| 12797 | 5' | -58.9 | NC_003387.1 | + | 6942 | 0.77 | 0.106945 |
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Target: 5'- uAUCGGGGU--GCGUgagggcacCCUGCuGGCCGCCc -3' miRNA: 3'- -UAGCCCCAuuCGCA--------GGACG-CUGGCGG- -5' |
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| 12797 | 5' | -58.9 | NC_003387.1 | + | 16558 | 0.77 | 0.098403 |
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Target: 5'- gAUCGGGGcggUGAGCaUCCgGcCGACCGCCu -3' miRNA: 3'- -UAGCCCC---AUUCGcAGGaC-GCUGGCGG- -5' |
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| 12797 | 5' | -58.9 | NC_003387.1 | + | 40147 | 1.1 | 0.000352 |
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Target: 5'- gAUCGGGGUAAGCGUCCUGCGACCGCCa -3' miRNA: 3'- -UAGCCCCAUUCGCAGGACGCUGGCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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