Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 12799 | 5' | -54.4 | NC_003387.1 | + | 40109 | 0.66 | 0.74976 |
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Target: 5'- cUCGcagGCGGCGg--CC-CGGCGUCGGg -3' miRNA: 3'- cAGCa--CGUUGUagaGGaGCCGCAGCU- -5' |
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| 12799 | 5' | -54.4 | NC_003387.1 | + | 43315 | 0.66 | 0.74976 |
|
Target: 5'- cGUCG-GCc-CA-CUCCUCGGCGaUCGu -3' miRNA: 3'- -CAGCaCGuuGUaGAGGAGCCGC-AGCu -5' |
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| 12799 | 5' | -54.4 | NC_003387.1 | + | 46469 | 0.74 | 0.305623 |
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Target: 5'- cUCGUGCAccgggaugaugacgGCgAUUUCCUCGGCGaCGAg -3' miRNA: 3'- cAGCACGU--------------UG-UAGAGGAGCCGCaGCU- -5' |
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| 12799 | 5' | -54.4 | NC_003387.1 | + | 47551 | 0.67 | 0.707143 |
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Target: 5'- gGUCGUGCu-CGUCggccgCaCUCGcGUGUCGGu -3' miRNA: 3'- -CAGCACGuuGUAGa----G-GAGC-CGCAGCU- -5' |
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| 12799 | 5' | -54.4 | NC_003387.1 | + | 48393 | 0.7 | 0.542323 |
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Target: 5'- -gCGcUGguGCGcCUUCUCGGCGUUGAg -3' miRNA: 3'- caGC-ACguUGUaGAGGAGCCGCAGCU- -5' |
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| 12799 | 5' | -54.4 | NC_003387.1 | + | 50303 | 0.66 | 0.770388 |
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Target: 5'- -gCGcUGCcACAUCUCCcaggCGGgGUCGu -3' miRNA: 3'- caGC-ACGuUGUAGAGGa---GCCgCAGCu -5' |
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| 12799 | 5' | -54.4 | NC_003387.1 | + | 50415 | 0.71 | 0.458933 |
|
Target: 5'- cGUCGgGCGGCAgaUCgucgCCUCGGCGUaCGc -3' miRNA: 3'- -CAGCaCGUUGU--AGa---GGAGCCGCA-GCu -5' |
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| 12799 | 5' | -54.4 | NC_003387.1 | + | 51417 | 0.74 | 0.315665 |
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Target: 5'- cGUCGU-CGACGUCgcccgCCagGGCGUCGAg -3' miRNA: 3'- -CAGCAcGUUGUAGa----GGagCCGCAGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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