Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 12799 | 5' | -54.4 | NC_003387.1 | + | 35507 | 0.67 | 0.685341 |
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Target: 5'- cGUCGaGCGGugUAUCUgCCUCGGCcUCGGc -3' miRNA: 3'- -CAGCaCGUU--GUAGA-GGAGCCGcAGCU- -5' |
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| 12799 | 5' | -54.4 | NC_003387.1 | + | 22488 | 0.68 | 0.663332 |
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Target: 5'- cGUCGUaccggGCGACGg--CCUCGGCGgcgcgcUCGAc -3' miRNA: 3'- -CAGCA-----CGUUGUagaGGAGCCGC------AGCU- -5' |
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| 12799 | 5' | -54.4 | NC_003387.1 | + | 47551 | 0.67 | 0.707143 |
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Target: 5'- gGUCGUGCu-CGUCggccgCaCUCGcGUGUCGGu -3' miRNA: 3'- -CAGCACGuuGUAGa----G-GAGC-CGCAGCU- -5' |
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| 12799 | 5' | -54.4 | NC_003387.1 | + | 50415 | 0.71 | 0.458933 |
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Target: 5'- cGUCGgGCGGCAgaUCgucgCCUCGGCGUaCGc -3' miRNA: 3'- -CAGCaCGUUGU--AGa---GGAGCCGCA-GCu -5' |
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| 12799 | 5' | -54.4 | NC_003387.1 | + | 19572 | 0.71 | 0.44898 |
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Target: 5'- aGUCGgcugGCGAgc-CUgCUCGGCGUCGAc -3' miRNA: 3'- -CAGCa---CGUUguaGAgGAGCCGCAGCU- -5' |
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| 12799 | 5' | -54.4 | NC_003387.1 | + | 26598 | 0.72 | 0.41986 |
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Target: 5'- cUCGUGCuGACAaCgCCUCGGCGgCGAa -3' miRNA: 3'- cAGCACG-UUGUaGaGGAGCCGCaGCU- -5' |
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| 12799 | 5' | -54.4 | NC_003387.1 | + | 31153 | 0.73 | 0.373967 |
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Target: 5'- aGUCGUGCAGgAUCagcucgguugCCUCGGUGcCGGg -3' miRNA: 3'- -CAGCACGUUgUAGa---------GGAGCCGCaGCU- -5' |
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| 12799 | 5' | -54.4 | NC_003387.1 | + | 40109 | 0.66 | 0.74976 |
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Target: 5'- cUCGcagGCGGCGg--CC-CGGCGUCGGg -3' miRNA: 3'- cAGCa--CGUUGUagaGGaGCCGCAGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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