Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12799 | 5' | -54.4 | NC_003387.1 | + | 26598 | 0.72 | 0.41986 |
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Target: 5'- cUCGUGCuGACAaCgCCUCGGCGgCGAa -3' miRNA: 3'- cAGCACG-UUGUaGaGGAGCCGCaGCU- -5' |
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| 12799 | 5' | -54.4 | NC_003387.1 | + | 3467 | 0.72 | 0.38287 |
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Target: 5'- gGUCGaUGC-GCGUCaCCcCGGCGUCGAg -3' miRNA: 3'- -CAGC-ACGuUGUAGaGGaGCCGCAGCU- -5' |
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| 12799 | 5' | -54.4 | NC_003387.1 | + | 31153 | 0.73 | 0.373967 |
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Target: 5'- aGUCGUGCAGgAUCagcucgguugCCUCGGUGcCGGg -3' miRNA: 3'- -CAGCACGUUgUAGa---------GGAGCCGCaGCU- -5' |
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| 12799 | 5' | -54.4 | NC_003387.1 | + | 35866 | 0.73 | 0.339781 |
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Target: 5'- -gCGUGCGGCGccaUCUCCUCGcGCGgcgUGAg -3' miRNA: 3'- caGCACGUUGU---AGAGGAGC-CGCa--GCU- -5' |
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| 12799 | 5' | -54.4 | NC_003387.1 | + | 51417 | 0.74 | 0.315665 |
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Target: 5'- cGUCGU-CGACGUCgcccgCCagGGCGUCGAg -3' miRNA: 3'- -CAGCAcGUUGUAGa----GGagCCGCAGCU- -5' |
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| 12799 | 5' | -54.4 | NC_003387.1 | + | 46469 | 0.74 | 0.305623 |
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Target: 5'- cUCGUGCAccgggaugaugacgGCgAUUUCCUCGGCGaCGAg -3' miRNA: 3'- cAGCACGU--------------UG-UAGAGGAGCCGCaGCU- -5' |
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| 12799 | 5' | -54.4 | NC_003387.1 | + | 6880 | 0.76 | 0.23839 |
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Target: 5'- cUCGcGCAGgAUCUCCgCGGCGUCGu -3' miRNA: 3'- cAGCaCGUUgUAGAGGaGCCGCAGCu -5' |
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| 12799 | 5' | -54.4 | NC_003387.1 | + | 37445 | 1.08 | 0.001356 |
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Target: 5'- uGUCGUGCAACAUCUCCUCGGCGUCGAc -3' miRNA: 3'- -CAGCACGUUGUAGAGGAGCCGCAGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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