Results 1 - 13 of 13 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
128 | 5' | -56.3 | AC_000006.1 | + | 14201 | 0.66 | 0.492798 |
Target: 5'- gGUGCUGCuGcCGGGCugcGGGGUGGacuuCACa -3' miRNA: 3'- -CACGGCG-C-GCCCG---UCCUACUaau-GUG- -5' |
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128 | 5' | -56.3 | AC_000006.1 | + | 20246 | 0.66 | 0.482149 |
Target: 5'- uUGuuGUGCuggaaGGGCAGGGUGAcgGCc- -3' miRNA: 3'- cACggCGCG-----CCCGUCCUACUaaUGug -5' |
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128 | 5' | -56.3 | AC_000006.1 | + | 21308 | 0.66 | 0.471612 |
Target: 5'- -cGCCGCGCuGGGgAGGGccacGUUGCGg -3' miRNA: 3'- caCGGCGCG-CCCgUCCUac--UAAUGUg -5' |
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128 | 5' | -56.3 | AC_000006.1 | + | 10872 | 0.66 | 0.461193 |
Target: 5'- cUGCCGguuucggGCGGGCAGGGagc-UGCGCg -3' miRNA: 3'- cACGGCg------CGCCCGUCCUacuaAUGUG- -5' |
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128 | 5' | -56.3 | AC_000006.1 | + | 17592 | 0.66 | 0.461193 |
Target: 5'- -aGCUGCcuucgguggaGCGGGCGuGGGUGGUggagGCAg -3' miRNA: 3'- caCGGCG----------CGCCCGU-CCUACUAa---UGUg -5' |
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128 | 5' | -56.3 | AC_000006.1 | + | 9244 | 0.67 | 0.450897 |
Target: 5'- -cGCCGcCGCGGGgA-GAUGAUcgaGCGCu -3' miRNA: 3'- caCGGC-GCGCCCgUcCUACUAa--UGUG- -5' |
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128 | 5' | -56.3 | AC_000006.1 | + | 10123 | 0.67 | 0.440728 |
Target: 5'- aUGuuGCGCaGcGGCAGGAaauaguccaUGGUcgGCACg -3' miRNA: 3'- cACggCGCG-C-CCGUCCU---------ACUAa-UGUG- -5' |
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128 | 5' | -56.3 | AC_000006.1 | + | 10687 | 0.67 | 0.42079 |
Target: 5'- -cGCCGgGgGGGUGGGAcGcAUUugGCg -3' miRNA: 3'- caCGGCgCgCCCGUCCUaC-UAAugUG- -5' |
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128 | 5' | -56.3 | AC_000006.1 | + | 20329 | 0.68 | 0.401406 |
Target: 5'- -gGCCGaugaGCGGGUAGGGgaagUugGCg -3' miRNA: 3'- caCGGCg---CGCCCGUCCUacuaAugUG- -5' |
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128 | 5' | -56.3 | AC_000006.1 | + | 8383 | 0.68 | 0.373418 |
Target: 5'- cGUGgCGCuCGGGCAGGucccgGUG-UUGCGCc -3' miRNA: 3'- -CACgGCGcGCCCGUCC-----UACuAAUGUG- -5' |
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128 | 5' | -56.3 | AC_000006.1 | + | 21758 | 0.7 | 0.275243 |
Target: 5'- aUGCaGCGCGGcGCGGGgAUGAUgcugaugcccgUGCACu -3' miRNA: 3'- cACGgCGCGCC-CGUCC-UACUA-----------AUGUG- -5' |
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128 | 5' | -56.3 | AC_000006.1 | + | 8349 | 0.71 | 0.231904 |
Target: 5'- -gGCCcCGCGGGCAGGGgcggcagagGCACg -3' miRNA: 3'- caCGGcGCGCCCGUCCUacuaa----UGUG- -5' |
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128 | 5' | -56.3 | AC_000006.1 | + | 12206 | 1.09 | 0.00039 |
Target: 5'- cGUGCCGCGCGGGCAGGAUGAUUACACc -3' miRNA: 3'- -CACGGCGCGCCCGUCCUACUAAUGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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