Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12803 | 5' | -55.4 | NC_003387.1 | + | 19335 | 0.66 | 0.710816 |
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Target: 5'- uGAAUGUgccUGcGGCGGCGUCCCAUc--- -3' miRNA: 3'- -CUUGCA---GCaUCGCCGCGGGGUAcaac -5' |
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| 12803 | 5' | -55.4 | NC_003387.1 | + | 22875 | 0.66 | 0.656111 |
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Target: 5'- cGACGaaUCGacAGgGGCGCCUCGUGUa- -3' miRNA: 3'- cUUGC--AGCa-UCgCCGCGGGGUACAac -5' |
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| 12803 | 5' | -55.4 | NC_003387.1 | + | 42142 | 0.66 | 0.656111 |
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Target: 5'- uGAACGUgGcGGCGGUGgCCCGcUGUc- -3' miRNA: 3'- -CUUGCAgCaUCGCCGCgGGGU-ACAac -5' |
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| 12803 | 5' | -55.4 | NC_003387.1 | + | 51308 | 0.67 | 0.64506 |
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Target: 5'- --uCGUCGgcGGCGGCGCCUCGc---- -3' miRNA: 3'- cuuGCAGCa-UCGCCGCGGGGUacaac -5' |
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| 12803 | 5' | -55.4 | NC_003387.1 | + | 38160 | 0.67 | 0.600834 |
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Target: 5'- aGAACGgccaGGCGGCGUCgaccgCCAUGUUGu -3' miRNA: 3'- -CUUGCagcaUCGCCGCGG-----GGUACAAC- -5' |
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| 12803 | 5' | -55.4 | NC_003387.1 | + | 43113 | 0.68 | 0.578845 |
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Target: 5'- cGAGCGcgaCGgcGCGGCGCCgCgGUGgUGg -3' miRNA: 3'- -CUUGCa--GCauCGCCGCGG-GgUACaAC- -5' |
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| 12803 | 5' | -55.4 | NC_003387.1 | + | 13906 | 0.69 | 0.49328 |
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Target: 5'- cGACGUCG-GGUGGCGCCCgAUc--- -3' miRNA: 3'- cUUGCAGCaUCGCCGCGGGgUAcaac -5' |
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| 12803 | 5' | -55.4 | NC_003387.1 | + | 37373 | 0.74 | 0.26726 |
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Target: 5'- --cUGUCGggcGGCGGCGCCCCGcUGUc- -3' miRNA: 3'- cuuGCAGCa--UCGCCGCGGGGU-ACAac -5' |
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| 12803 | 5' | -55.4 | NC_003387.1 | + | 35794 | 1.08 | 0.001055 |
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Target: 5'- cGAACGUCGUAGCGGCGCCCCAUGUUGu -3' miRNA: 3'- -CUUGCAGCAUCGCCGCGGGGUACAAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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