Results 61 - 67 of 67 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12805 | 5' | -58 | NC_003387.1 | + | 27820 | 0.66 | 0.598485 |
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Target: 5'- -aACGCaCUUGCCggugccGUCGGUggacaGGGCg -3' miRNA: 3'- ggUGCG-GAACGGgaa---CAGCCAg----CCCG- -5' |
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| 12805 | 5' | -58 | NC_003387.1 | + | 1577 | 0.66 | 0.598485 |
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Target: 5'- -aGCGCCUaacGCCCUgg-CGGUCGacaugucgcacGGCc -3' miRNA: 3'- ggUGCGGAa--CGGGAacaGCCAGC-----------CCG- -5' |
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| 12805 | 5' | -58 | NC_003387.1 | + | 22959 | 0.66 | 0.598485 |
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Target: 5'- aCCGCGUggggcGCCCcgGUCaucguGUCGGGCg -3' miRNA: 3'- -GGUGCGgaa--CGGGaaCAGc----CAGCCCG- -5' |
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| 12805 | 5' | -58 | NC_003387.1 | + | 694 | 0.66 | 0.605971 |
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Target: 5'- aCGCaggGCCUUGaucgCCUcGUCgucgagggccgacaGGUCGGGCa -3' miRNA: 3'- gGUG---CGGAACg---GGAaCAG--------------CCAGCCCG- -5' |
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| 12805 | 5' | -58 | NC_003387.1 | + | 13076 | 0.66 | 0.609183 |
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Target: 5'- cCCACGCCaUGCgCgacaccgGGUCGaGGCu -3' miRNA: 3'- -GGUGCGGaACGgGaacag--CCAGC-CCG- -5' |
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| 12805 | 5' | -58 | NC_003387.1 | + | 12271 | 0.66 | 0.609183 |
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Target: 5'- cCCGCGCCUgGCgaCCggcgGcCGGaucagCGGGCc -3' miRNA: 3'- -GGUGCGGAaCG--GGaa--CaGCCa----GCCCG- -5' |
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| 12805 | 5' | -58 | NC_003387.1 | + | 26186 | 0.66 | 0.610254 |
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Target: 5'- gUCGCGUgCUUGCCUgacggcucgucgucGUCGGccucgcacuccUCGGGCa -3' miRNA: 3'- -GGUGCG-GAACGGGaa------------CAGCC-----------AGCCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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