Results 61 - 67 of 67 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 12805 | 5' | -58 | NC_003387.1 | + | 20457 | 0.68 | 0.482228 |
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Target: 5'- cCCACGCCUUGUcggCCUcGgcgacggccaggCGGUCGGccaGCu -3' miRNA: 3'- -GGUGCGGAACG---GGAaCa-----------GCCAGCC---CG- -5' |
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| 12805 | 5' | -58 | NC_003387.1 | + | 30235 | 0.67 | 0.491214 |
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Target: 5'- uUCGCGCCagUUGgCCgggucGUCGGggucggcgccggggUCGGGCa -3' miRNA: 3'- -GGUGCGG--AACgGGaa---CAGCC--------------AGCCCG- -5' |
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| 12805 | 5' | -58 | NC_003387.1 | + | 18968 | 0.67 | 0.511451 |
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Target: 5'- gCgGCGCCgccgUUGCCCUUGcCGGggugccgcuuagacgGGGCc -3' miRNA: 3'- -GgUGCGG----AACGGGAACaGCCag-------------CCCG- -5' |
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| 12805 | 5' | -58 | NC_003387.1 | + | 38716 | 0.67 | 0.524789 |
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Target: 5'- gUACGCCggguUGCCCUgccagucauUCGGauacUUGGGCg -3' miRNA: 3'- gGUGCGGa---ACGGGAac-------AGCC----AGCCCG- -5' |
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| 12805 | 5' | -58 | NC_003387.1 | + | 46904 | 0.67 | 0.524789 |
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Target: 5'- gCCcCGCCUUGCgCgggGUugcgcuucggUGGUgCGGGCg -3' miRNA: 3'- -GGuGCGGAACGgGaa-CA----------GCCA-GCCCG- -5' |
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| 12805 | 5' | -58 | NC_003387.1 | + | 45547 | 0.67 | 0.535139 |
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Target: 5'- aCC-CGCUUgaucgGCCCcgguguuggUGUCGGgacCGGGUa -3' miRNA: 3'- -GGuGCGGAa----CGGGa--------ACAGCCa--GCCCG- -5' |
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| 12805 | 5' | -58 | NC_003387.1 | + | 26186 | 0.66 | 0.610254 |
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Target: 5'- gUCGCGUgCUUGCCUgacggcucgucgucGUCGGccucgcacuccUCGGGCa -3' miRNA: 3'- -GGUGCG-GAACGGGaa------------CAGCC-----------AGCCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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